Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Rice Science》 2017-01
Add to Favorite Get Latest Update

Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heatsensitive Lines Under High Night Temperature Stress at Filling Stage

HUANG Xiaoping;ZHANG Hongyu;LEI Gang;WANG Zhimei;ZHANG Zhi;HE Chao;LIAO Jianglin;HUANG Yingjin;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University;Key Laboratory of Agriculture Responding to Climate Change,Jiangxi Agricultural University;  
【Objective】To understand molecular mechanism of rice tolerance to high night temperature at filling stage,we identify the differentially expressed proteins(DEPs) and screen the proteins involving in regulating the heat-tolerance in rice.【Method】The heat-tolerant rice line XN0437 T and the heat-sensitive rice line XN0437 S originated from a near-genetic lines were used as plant materials.Rice was pot-cultivated under traditional management.To ensure that only grain samples with uniform growth were used for proteomes analysis,rice panicle bloomed on the same date were labeled with same markers and spikelets flowering on the same date on the labeled panicle were further labeled.On the 8th day after flowering,rice plants with same label were transferred to chambers for high night temperature treatment.Rice grains with same label were harvested and total proteins of the grain samples were extracted after high temperature treatment.The 8-plex i TRAQ kits combined with the LC-MS/ MS technology were used to analyze the DEPs between the heat-tolerant and heat-sensitive lines.【Result】After protein's databases searching and differentially expressed proteins analysis,3130 proteins were finally identified and 36 proteins showed differential expression levels between the heat-tolerant and heat-sensitive line.For the 36 DEPs,14 proteins(38.9%) had functional annotation,12(33.3%) had putative function,and 10(33.3%) were functional unknown proteins.The 14 function-known proteins were involved in energy metabolism(five proteins),transport and metabolism(three proteins),photosynthesis(two proteins) and defense response(four proteins).【Conclusion】High night temperature impacts the expressed patterns of the proteins involved in energy metabolism,matter transport and metabolism,photosynthesis and defense response in rice at filling stage.We suggested that up-regulated expression of the zinc finger proteins(Q67TK9 and Q10N88),and down-regulated expression of the zinc finger protein(Q5YLY5) in rice grain could enhance the heat-tolerance in rice.
【Fund】: 江西省青年科学基金重点资助项目(20133ACB21004);; 国家自然科学基金资助项目(31260315 31471467)
【CateGory Index】: S511
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Linghua;CHENG Zuxin;XU Ming;ZHENG Jingui;Jinshan College,Fujian Agriculture and Forestry University;College of Crop Science,Fujian Agriculture and Forestry University;;Research Advance in iTRAQ Technology and Its Application in Proteomics of Rice[J];中国农业科技导报;2017-12
【Citations】
Chinese Journal Full-text Database 7 Hits
1 ZHANG Gui-Lian;LIAO Bin;WU Xiao-Jin;XIAO Ying-Hui;XIAO Lang-Tao;CHEN Li-Yun;College of Agronomy,Hunan Agricultural University;Post-Doctoral Innovation Base of Agricultural Science and Technology Promotion Center of Longgang District,Shenzhen;Hunan Provincial Key Laboratory of Phytohormones and Growth Development,Hunan Agricultural University;;Effect of High Temperature on Activities of Enzymes Associated with Starch Synthesis and Hormones Contents in Endosperm of Rice[J];植物生理学报;2014-12
2 LIAO Jiang-lin;SONG Yu;ZHONG Ping-an;ZHOU Hui-wen;ZHANG Hong-yu;HUANG Ying-jin;Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University;Key Laboratory of Agriculture Responding to Climate Change, Jiangxi Agricultural University;;Identification of the Differentially Expressed Proteins Between Heat-Tolerant and Heat-Sensitive Rice Responding to High-Temperature Stress at the Early Milky Stage[J];中国农业科学;2014-16
3 LIAO Jiang-lin,ZHANG Hong-yu,SHAO Xue-lian,ZHONG Ping-an,HUANG Ying-jin(Key Laboratory of Crop Physiological Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,China);Identification for Heat Tolerance in Backcross Recombinant Lines and Screening of Backcross Introgression Lines with Heat Tolerance at Milky Stage in Rice[J];Rice Science;2011-04
4 Jiang-Lin Liao and Ying-Jin Huang * MOE Key Laboratory of Crop Physiological Ecology and Genetic Breeding, Jiangxi Agricultural University, Nanchang 330045, China.;Evaluation of Protocols Used in 2-D Electrophoresis for Proteome Analysis of Young Rice Caryopsis[J];Genomics, Proteomics & Bioinformatics;2011-06
5 XIE Xiao-jin1,2,LI Bing-bai2,WANG Lin1,DAI Qin-ru1,SHEN Shuang-he1 (1.Colloge of Applied Meteorology,Nanjing University of Information Science and Technology,Nanjing 210044,China;2.Institute of Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing 210044);Spatial and Temporal Distribution of High Temperature and Strategies to Rice Florescence Harm in the Lower-middle Reaches of Yangtze River[J];中国农业气象;2010-01
6 ZHOU Zhu Qing;LAN Sheng Yin;XU Zhen Xiu;YANG Ze Min College of life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Postdoctoral Work Station of Biology of Huazhong Agricultural University, Wuhan 430070, China;DYNAMIC CHANGE OF ATPase ACTIVITY ON AMYLOPLASTS AND PROTEIN BODIES DURING THE ENDOSPERM DEVELOPMENT IN RICE(Oryza sative L.)[J];实验生物学报;2005-01
7 WEI Cun xu, LAN Sheng yin, XU Zhen xiu (College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070);Ultracytochemical Localization and Functional Analysis of ATPase During the Endosperm Development in Oryza sativa L.[J];中国农业科学;2003-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HE Weihui;LIU Xulin;WANG Jin;Wugang Meteorological Observatory;;Analysis on the meteorological Factors of the Severe Yield Reduction of Wugang's 2014 Early Season Rice——Based by the Data Comparison on the Meteorological Factors[J];邵阳学院学报(自然科学版);2017-06
2 GUO Jian-mao;WU Yue;YANG Shen-bin;JIANG Xiao-dong;XIE Xiao-yan;WANG Jin-jie;SHEN Shuang-he;Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disaster/Jiangsu Key Laboratory of Agricultural Meteorology, Nanjing University of Information Science & Technology;College of Applied Meteorology,Nanjing University of Information Science & Technology;;Yield Differences and its Causes for One Season Rice Under Different Sowing Dates in Typical High Temperature Year[J];中国农业气象;2017-02
3 HUANG Xiaoping;ZHANG Hongyu;LEI Gang;WANG Zhimei;ZHANG Zhi;HE Chao;LIAO Jianglin;HUANG Yingjin;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University;Key Laboratory of Agriculture Responding to Climate Change,Jiangxi Agricultural University;;Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heatsensitive Lines Under High Night Temperature Stress at Filling Stage[J];中国水稻科学;2017-01
4 YANG Huan;SHEN Xin;DING Meng-qiu;LU Da-lei;LU Wei-ping;Yangzhou University, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops;;Effects of High Temperature after Pollination on Grain Development and Endogenous Hormone Contents of Waxy Maize[J];玉米科学;2017-02
5 LAN Xu;GU Zheng-dong;DING Yan-fei;WANG Ke;JIANG Qiong;ZHU Cheng;College of Life Science,China Jiliang University/Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province;;Effect of High Temperature Stress on Physiological Characteristics of Spikelet of Rice During Flowering Stage[J];中国水稻科学;2016-06
6 Zhao Yanan;Wang Ying;Zhang Dongjie;Shen Yan;Yang Yijie;College of Food Science,Heilongjiang Bayi Agricultural Universitiy;National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural Universitiy;;Fingerprint Construction of Rice DNA Based on the SSR Molecular Marker Technology[J];黑龙江八一农垦大学学报;2016-05
7 WANG Chun-yi;YAO Peng-juan;ZHANG Ji-quan;REN Yi-fang;Chinese Academy of Meteorological Sciences;School of Environment,Natural Disaster Research Institute,Northeast Normal University;Jiangsu Meteorologic Bureau;;Risk Assessment of Cold and Hot Damages for Double-Cropping Early Rice(DCER) in Lower-Middle Reaches of the Yangtze River Basin[J];中国农业科学;2016-13
8 PENG Bo;PANG Rui-hua;SUN Yan-fang;LI Hui-long;SONG Xiao-hua;ZHOU Qi-ying;SONG Shi-zhi;LI Liang-hao;JIANG Yi-dan;YUAN Hong-yu;College of Life Science,Xinyang Normal University;Academy of Agricultural Biological Resources Protection and Utilization in Dabie Mountain,Xinyang Normal University;Xinyang Academy of Agricultural Sciences in Henan Province;;Research Progress in Starch Synthesis and Its Regulation in Rice Seeds[J];江西农业学报;2016-06
9 Geng Xinmin;Yang Qiuyu;Zheng Fuchao;Wang Lianggui;Xie Weijia;College of Landscape Architecture, Nanjing Forestry University;Flower Research Institute, Yunnan Academy of Agricultural Sciences;;Differences in the Protein Expression of Four Rhododendron Species Seedlings under High Temperature Stress[J];分子植物育种;2016-06
10 WANG Ya-Liang;ZHANG Yu-Ping;ZHU De-Feng;XIANG Jing;WU Hui;CHEN Hui-Zhe;ZHANG Yi-Kai;China National Rice Research Institute/State Key Laboratory of Rice Biology;;Effect of Heat Stress on Spikelet Degeneration and Grain Filling at Panicle Initiation Period of Rice[J];作物学报;2016-09
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 HUANG Xiaoping;ZHANG Hongyu;LEI Gang;WANG Zhimei;ZHANG Zhi;HE Chao;LIAO Jianglin;HUANG Yingjin;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University;Key Laboratory of Agriculture Responding to Climate Change,Jiangxi Agricultural University;;Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heatsensitive Lines Under High Night Temperature Stress at Filling Stage[J];中国水稻科学;2017-01
2 TANG Cheng;CHEN Lu;AN Min-min;MENG Dan;YANG Li-ming;LUO Yu-ming;Hi-tech Innovation Center in Huaian City;Jiangsu Key Laboratory for Eco-Agricultural and Biotechnology Around Hongze Lake/School of Life Sciences,Huaiyin Normal University;School of Life Science,Nanjing Normal University;;Proteomic Analysis Reveals an Intimate Protein Pathways Provoked by Blast in Rice Seedling Leaves[J];淮阴师范学院学报(自然科学版);2014-04
3 Zhang Genlian Fan Shuli Song Meizhen Pang Chaoyou Yu Shuxun (Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang 455000);Development of Plant Proteomics Research Technology[J];生物技术通报;2011-07
4 XIE Xiu-Zhi 1),WANG Xin 2),LIU Li-Hua 1),DONG Shi-Lei 3),PI Xiong-E2),LIU Wei 2)(1) School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010051,China;2)Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3)School of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,China);ITRAQ Technology and Its Application in Proteomics[J];中国生物化学与分子生物学报;2011-07
5 LUO Zhi-wen ZHU Liang XIE Wei-fen(Department of Gastroenterology,Chang-zheng Hospital,the Second Military Medical University, Shanghai 200003,China);Advances in Isobaric Tags for Relative and Absolute Quantitation Techniques Research[J];中国生物工程杂志;2006-10
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Jian-xia;ZHANG Yu-ping;ZHU De-feng;LIN Xian-qing;XIANG Jing;CHEN Hui-zhe;HU Sheng-bo;State Key Laboratory of Rice Biology,China National Rice Research Institute;;Influence of Flowering Characteristics on Spikelet Fertility under High Temperature[J];中国水稻科学;2014-03
2 ZHANG Gui-lian,ZHANG Shun-tang,WANG Li,XIAO Ying-hui,TANG Wen-bang,CHEN Guang-hui,CHEN Li-yun(College of Agronomy,Hunan Agricultural University,Changsha 410128);Effects of High Temperature at Different Times During the Heading and Filling Periods on Rice Quality[J];中国农业科学;2013-14
3 LIAO Jiang-Lin 1 , XIAO Xiao-Jun 1,2 , SONG Yu 1 , ZHOU Qing-Hong 1 , HUANG Ying-Jin 1,* 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; 2 The Red Soil Research Institute of Jiangxi Province, Nanchang 331717, China;Effects of High Temperature on Grain-Filling of Rice Caryopsis and Physi-ological and Biochemical Characteristic of Flag Leave at Early Milky Stage[J];植物生理学报;2013-02
4 GAO Huan-Ye1,2,WANG San-Gen1,ZONG Xue-Feng1,TENG Zhong-Hua1, ZHAO Fang-Ming1,LIU Zhao1(1.College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China; 2.College of Agronomy,Guizhou University,Guiyang 550025,China);Effects of combined high temperature and drought stress on amylose and protein contents at rice grain-filling stage[J];中国生态农业学报;2012-01
5 LIAO Jiang-lin,ZHANG Hong-yu,SHAO Xue-lian,ZHONG Ping-an,HUANG Ying-jin(Key Laboratory of Crop Physiological Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,China);Identification for Heat Tolerance in Backcross Recombinant Lines and Screening of Backcross Introgression Lines with Heat Tolerance at Milky Stage in Rice[J];Rice Science;2011-04
6 Jiang-Lin Liao and Ying-Jin Huang * MOE Key Laboratory of Crop Physiological Ecology and Genetic Breeding, Jiangxi Agricultural University, Nanchang 330045, China.;Evaluation of Protocols Used in 2-D Electrophoresis for Proteome Analysis of Young Rice Caryopsis[J];Genomics, Proteomics & Bioinformatics;2011-06
7 HUANG Ming 1,2 ,XIE Fang-min 2 ,CHEN Li-yun 1 ,ZHAO Xiang-qian 3 ,L.JOJEE 2 ,D.MADONNA 2 ( 1 Rice Research Institute,Hunan Agricultural University,Changsha 410128,China; 2 Plant Breeding,Genetics and Biotechnology Division,International Rice Research Institute,Metro Manila,DAPO Box 7777,Philippines; 3 Grain Quality,Nutrition and Postharvest Center,International Rice Research Institute,Metro Manila,DAPO Box 7777,Philippines);Comparative Analysis of Genetic Diversity and Structure in Rice Using ILP and SSR Markers[J];Rice Science;2010-04
8 TENG Zhonghua1,ZHI Li2,L Jun1,ZONG Xuefeng1,WANG Sangen1,*,HE Guanghua1 1 College of Agronomy and Biotechnology,Southwest University,400716 Chongqing,China 2 College of Horticulture and Landscape Architecture,Southwest University,400716 Chongqing,China;Effects of high temperature on photosynthesis characteristics,phytohormones and grain quality during filling-periods in rice[J];生态学报;2010-23
9 ZHANG Chun-hong1,LI Jin-zhou1,2,ZHU Zhen1,ZHANG Ya-dong1,ZHAO Ling1,WANG Cai-lin1,(1Institute of Food Crops,Jiangsu High Quality Rice R & D Center,Nanjing Branch of Chinese National Center for Rice Improvement,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China);Cluster Analysis on Good Eating Quality japonica Rice (Oryza sativa) Based on SSR Markers and Phenotypic Traits[J];中国水稻科学;2009-06
10 XIE Xiao-jin1,2,LI Bing-bai2,SHEN Shuang-he1,LIU Chun-lei1,TANG Ri-sheng2,CHENG Gao-feng2(1.Colloge of Applied Meteorology,Nanjing University of Information Science and Technology,Nanjing 210044,China;2.Institute of Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014);Influence of High Temperature Stress on Some Physiological Characteristics of Flag Leaves of Rice Variety Yangdao 6[J];中国农业气象;2009-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved