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《Chinese Journal of Rice Science》 2019-01
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Identification and Gene Mapping of a white-stripe leaf after transplanting at low temperature Mutant in Rice

LIN Tianzi;SUN Liting;GONG Hongbin;WANG Yihua;LIU Linglong;ZHAO Zhigang;JIANG Ling;WAN Jianmin;State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University/Key Laboratory of Biology, Genetics and Breeding of japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture/The Yangtze River Valley Hybrid Rice Collaboration Innovation Center/Jiangsu Collaboration Innovation Center for Modern Crop Production;Zhenjiang Agricultural Research Institute;  
【Objective】 Isolation and characterization of leaf-color mutation related genes lays a firm theoretical foundation for dissecting the molecular mechanism underlying chloroplast development, chlorophyll biosynthesis, and photosynthesis in rice. 【Method】A spontaneous leaf-color mutant, termed as white stripe leaf after transplanting at low-temperature(wltt), was obtained from the progeny of japonica cultivar Zhennuo 19. The main agronomic traits of the wild type and wltt were determined at maturity. The pigment contents and ultrastructure of chloroplast of newly emerged leaves were analyzed at the seedling stage, fifteen days after transplanting, at the tillering stage under direct seeding. Genetic analysis was carried out by reciprocal cross of the wild type and wltt. An F_2 population derived from the cross wltt×9311 was used for gene mapping. Quantitative RT-PCR was carried out to analyze the relative expression of genes associated with chloroplast development and chlorophyll biogenesis in the wild type and the wltt mutant. 【Result】The white-striped leaves in the wltt mutant only emerged at 15 days after transplanting at low temperature such as 20℃. No white-striped leaf was observed under direct seeding treatment. However, leaves of the mutant developed normally at the late tillering stage. Simulation experiments showed that the mutant phenotype was caused by root injury at low temperature. Compared with the wild type, the pigment contents in white-stripe leaves of the wltt mutant were significantly decreased, accompanying by reduced photosynthetic rate. Simultaneously, most of the mesophyll cells had no chloroplasts. The expression levels of genes associated with chloroplast development, chlorophyll biosynthesis, and photosynthetic system were all down-regulated in the mutant. At maturity, the mutant was featured with reduced plant height, panicle length, flag leaf length and number of spikelets per panicle relative to its wild type. Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. Moreover, the WLTT gene was mapped within an 853 kb region near the centromere on chromosome 2, between InDel markers L22 and L26, in which no gene related to leaf color was reported. 【Conclusion】WLTT is a key gene regulating leaf color after transplanting at low temperature, which plays an important role in chloroplast development.
【Fund】: 国家重点研发计划资助项目(2017YFD0100400);; 江苏省重点研发计划资助项目(BE2015363);; 江苏省农业科技自主创新资金资助项目[CX(16)1029]
【CateGory Index】: S511
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 TAN Yan-Ning;SUN Xue-Wu;YUAN Ding-Yang;SUN Zhi-Zhong;YU Dong;HE Qiang;DUAN Mei-Juan;DENG Hua-Feng;YUAN Long-Ping;College of Agronomy, Hunan Agricultural University;State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center;State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center, Changsha 410125, China;;Identification and Fine Mapping of Green-Revertible Chlorina Gene grc2 in Rice(Oryza sativa L.)[J];作物学报;2015-06
2 LI Yan-qun;ZHONG Ping;GAO Zhi-yan;ZHU Bai-yang;CHEN Dan;SUN Chang-hui;WANG Ping-rong;DENG Xiao-jian;Rice Research Institute, Sichuan Agricultural University;;Morphological Characterization and Candidate Gene Analysis of Zebra Leaf Mutant zebra524 in Rice[J];中国农业科学;2014-15
3 XIA Jia-Ping, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, LI Jun-Hui, andLIU Lu-Xiang*Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China;Differential Expression of Chloroplast Genes in Chlorophyll-Deficient Wheat Mutant Mt135 Derived from Space Mutagenesis[J];作物学报;2012-11
4 LI Xianyong,WANG Chutao,LI Shunwu,HE Yongxin,CHEN Shiquan(Chongqing Institute of Agricultural Sciences,Banan Chongqing 400055,China);The discovery of a high chlorophyll content gene in rice[J];西南农业学报;2002-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Ya-qin;SHI Jun-qiong;ZHANG Ting;LI Yan;ZHANG Tian-quan;ZHANG Xiao-long;SANG Xian-chun;LING Ying-hua;HE Guang-hua;Rice Research Institute of Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops;;Characterization and Candidate Gene Analysis of Yellow-Green Leaf Mutant ygl13 in Rice(Oryza sativa)[J];中国农业科学;2015-21
2 LING Ying-hua;SHI Jun-qiong;LIU Zhong-xian;SANG Xian-chun;YANG Zheng-lin;ZHANG Chang-wei;ZHAO Fang-ming;HE Guang-hua;Rice Research Institute of Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops;;Identification and Gene Mapping of a Yellow-Green Leaf Mutant ylg4(t) in Rice(Oryza sativa L.)[J];西南大学学报(自然科学版);2015-10
3 HE Ni-Qing;LIU Zhou;ZHANG Long;BAI Su-Yang;TIAN Yun-Lu;JIANG Ling;WAN Jian-Min;State Key Laboratory of Crop Genetics and Germplasm Enhancement / Research Center of Jiangsu Plant Gene Engineering / Ministry of Agriculture Key Laboratory of Biology / Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River / The Yangtze River Valley Hybrid Rice Collaboration Innovation Center, Nanjing Agricultural University;;Genetic Analysis of a New Yellow-green Leaf Mutant and Fine-mapping of Mutant Gene in Rice[J];作物学报;2015-08
4 DONG Qing;ZHANG Ying-xin;ZHANG Zhen-hua;ZHOU Quan;QIN Ya-zhi;WANG Hong;CHENG Shi-hua;CAO Li-yong;SHEN Xi-hong;China National Rice Research Institute/National Center for Rice Improvement;Key Laboratory for Zhejiang Super Rice Research;Rice Research Institute/Key Laboratory of Southwest Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University;;Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant w390 in Rice(Oryza sativa L.)[J];中国水稻科学;2015-03
5 Zeng Zhaosong;Zeng Xiaofang;Zhao Degang;Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Guizhou University),Ministry of Education;Guizhou Key Lab of Agro-bioengineering (Guizhou University);Collage of Life Sciences,Guizhou University;The Stdte Key Laboratory of Green Pesticide and Agricultural Bioengineering (Guizhou University),Ministry of Education;;The Photosynthetic Characteristics of Leaf Color Mutants of Guizhou landrace Cultivar Rice Lailong[J];分子植物育种;2014-05
6 JIANG Shao-hua1,ZHOU Hua1,LIN Dong-zhi1,*,DONG Yan-jun1,*,YE Sheng-hai2,ZHANG Xiao-ming2(1Laboratory of Plant Genetics and Functional Genes,College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China;2Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China);Identification and Gene Mapping of a Thermo-sensitive Leaf-color Mutant at Seedling Stage in Rice[J];中国水稻科学;2013-04
7 TIAN Feng,ZHANG Jing-ya,SUN De-ren,CHEN Gui-song,HUA Qing,GUO Ai-guang,XU Hong(College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China);Expression Profiles of Chloroplast Genome in Wheat Seedlings of Albinism Strain and Related Materials[J];麦类作物学报;2013-04
8 GE Shaoxing,MA Xiaojing,LIN Dongzhi,DONG Yanjun*(College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China);Genetic analysis and preliminary gene mapping of a novel seeding yellow-leaf mutant in rice[J];上海师范大学学报(自然科学版);2013-01
9 LI Yu-hong1, 2, WANG Bao-he2, DAI Zheng-yuan2, LI Ai-hong2, LIU Guang-qing1, ZUO Shi-min1, ZHANG Hong-xi1, 2, PAN Xue-biao1 (1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2Lixiahe Region Agricultural Research Institute of Jiangsu Province, Nanjing Subcenter of National Rice Improvement Center, Yangzhou 225007, China);Morphological Structure and Genetic Mapping of New Leaf-Color Mutant Gene in Rice (Oryza sativa)[J];Rice Science;2012-02
10 LIU Bao-fu1 CHEN Xi-feng1 JIN Yang1 GU Zhi-min1 ZHU Xu-dong2 MA Bo-jun1(1.College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004; 2.State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou,Zhejiang 310006);IDENTIFICATION AND GENETIC ASSAY OF A HIGH-CHLOROPHYLL-CONTENT MUTANT IN RICE[J];核农学报;2011-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HE Zhizhou;YIN Ming;XIE Zhenyu;WANG Yue;SHEN Jiankai;LI Liping;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences;College of Agronomy,Hunan Agricultural University;;Genetic Analysis and Breeding Application of a Novel Rice Mutant with Virescent Yellow Leaves[J];热带作物学报;2013-11
2 GUO Tao;HUANG Yong-Xiang;LUO Wen-Long;HUANG Xuan;WANG Hui;CHEN Zhi-Qiang;LIU Yong-Zhu;South China Agricultural University,National Engineering Research Center of Plant Space Breeding;;Gene Differential Expression of a Green-Revertible Albino and High-Tillering Dwarf Mutant hfa-1 by Using Rice Microarray[J];作物学报;2013-12
3 WANG Ping-rong,WANG Bing,SUN Xiao-qiu,SUN Chang-hui,WAN Chun-mei,MA Xiao-zhi,DENG Xiao-jian(Rice Research Institute,Sichuan Agricultural University,Chengdu 611130);Fine Mapping and Physiological Characteristics of A Green-Revertible Albino Gene gra75 in Rice[J];中国农业科学;2013-02
4 GUO Tao,HUANG Yong-Xiang,HUANG Xuan,LIU Yong-Zhu,ZHANG Jian-Guo,CHEN Zhi-Qiang,and WANG HuiSouth China Agricultural University /National Engineering Research Center of Plant Space Breeding,Guangzhou 510642,China;Map-Based Cloning of a Green-Revertible Albino and High-Tillering Dwarf Gene hw-1(t) in Rice[J];作物学报;2012-08
5 CHENG Hong-liang1,CHEN Jia-fa1,DING Jun-qiang1,WU Jian-yu2(1.Agronomy College of Henan Agricultural University,Zhengzhou 450002,China; 2.Life Sciences College of Henan Agricultural University,Zhengzhou 450002,China);Genetic Analysis and Gene Mapping of a Leaf Mutant in Maize[J];华北农学报;2011-03
6 JIANG Yuan1,HE Yun1,FAN Shu-li2,YU Jia-ning1,SONG Mei-zhen2 (1.College of Life Science,Shaanxi Normal University,Xi'an,Shaanxi 710062,China;2.Cotton Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Cotton Genetics Improvement,MOA,Anyang,Henan 455000,China);The Identification and Analysis of RNA Editing Sites of 10 Chloroplast Protein-coding Genes from Virescent Mutant of Gossypium hirsutum[J];棉花学报;2011-01
7 ZHAO Hong-Bing,GUO Hui-Jun,ZHAO Lin-Shu,GU Jia-Yu,ZHAO Shi-Rong,LI Jun-Hui,and LIU Lu-Xiang Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement,Beijing 100081,China;Agronomic Traits and Photosynthetic Characteristics of Chlorophyll-Deficient Wheat Mutant Induced by Spaceflight Environment[J];作物学报;2011-01
8 GUO Shi-wei1,WANG Yong-fei2,MA San-mei2,LI Xia1,GAO Dong-ying1 (1 Institute of Food Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center,Nanjing 210014,China;2Department of Bioengineering,Jinan University,Guangzhou 510632,China);Genetic Analysis and Fine Mapping of a Green-Revertible Albino Leaf Mutant in Rice[J];中国水稻科学;2011-01
9 ZHAO Hong-bing GUO Hui-jun ZHAO Lin-shu GU Jia-yu ZHAO Shi-yu LI Jun-hui LIU Lu-xiang(National Center of Space Mutagenesis for Crop Improvement National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science,Chinese Academy of Agricultural Sciences/Beijing 100081);A TEMPERATURE-SENSITIVE WINTER WHEAT CHLOROPHYLL MUTANT DERIVED FROM SPACE MUTAGENESIS[J];核农学报;2010-06
10 ZHANG XiangQian1,LI XiaoYan2,ZHU HaiTao1,WANG Tao1 & XIE XinMing1 1 College of Agriculture,South China Agricultural University,Guangzhou 510642,China;2 College of Agronomy,Northwest A&F University,Yangling 712100,China;Identification and candidate gene analysis of stage green-revertible albino mutant in rice(Oryza sativa L.)[J];科学通报;2010-23
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