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《Chinese Journal of Rice Science》 2019-01
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Screening Reference Genes and Evaluating of Their Expression Stability for qRT-PCR Normalization in Chilo suppressalis(Lepidoptera: Pyralididae)

XU Hongxing;WANG Guorong;LU Yanhui;YANG Yajun;ZHENG Xusong;TIAN Junce;Lü Zhongxian;Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture, P. R.of China/Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences;Xiaoshan Agricultural Technology Extension Center;  
【Objective】We aim to screen reference genes stably expressed in striped stem borers(SSB), Chilo suppressalis, under certain conditions, to lay a foundation for the study on the gene expression in SSB. 【Method】According to the results of SSB transcriptome data, 11 candidate genes from SSB were selected and their expression stability was detected under different treatment conditions(including development duration, tissues, feeding on different diets and rice varieties, dsRNA and mixed samples under temperature stress, insecticide treatment), by using real-time fluorescence quantitative PCR. The stability of the 11 candidate genes was evaluated by RefFinder, BestKeeper, GeNorm, NormFinder and ΔC_(t~.) 【Result】AK, RPL10 and EF1 were highly stable in different development durations; EF1, TUB and ACTB were highly stable in different tissues. TUB, RPL10 and EF1 were relatively stable under temperature stress. TF4 and ACTA were stable in insecticide treated samples. TUB, TF4, EF1 and RPL10 were stable in samples fed on different diets. TUB and EF1 were stable reference genes in samples fed on different rice varieties. TUB, AK, ACTB and EF1 were stable in the sample treated with dsRNA. EF1, TUB and ACTB were stable in mixed samples. 【Conclusion】 It provide a reference for selection of suitable reference gene under different test conditions, and also provide more reliable and accurate data for research in gene expression in rice SSB.
【Fund】: 国家自然科学基金资助项目(31672050);; 浙江省植物有害生物防控省部共建国家重点实验室培育基地自主设计项目(2010DS700124-ZZ1601)
【CateGory Index】: S435.112.1
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