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Identification of Rotundine Metabolites in Rat Bile by HPLC/MS/MS

MOU Yun-Ya,YU Bo-Yang* Department of Chinese Prescription,China Pharmaceutical University,Nanjing 210038,China  
AIM:To investigate the main metabolites of rotundine in rat bile.METHOD:Bile of rats after oral administration of rotundine(200 mg·kg-1)was analysed by HPLC/MS/MS.Chromatographic analysis was performed on a Lichrospher-C 18 column(5 μm,4.6 mm ×250 mm);mobile phase:methanol-water-triethylamine(37630.063,V/V/V),adjusting pH to 6.3 with acetic acid.UV detector was set at 281*!nm and positive ion mode was operated.RESULT:Two metabolites were found in rat bile,whose structure may be glucuronide conjugates of rotundine after demethylation.CONCLUSION:Conjugate with glucuronic acid after demethylation may be the main excretion pathway of rotundine in rat bile.
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