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Fingerprint Analysis of Supercritical Fluid CO_2 Extract of Origanum vulgare L.by GC

WANG Guang-Zhong,SUN Li-Juan,LIU Hong-Bing,XU Han-Lin,LIU Yan-Wen*Hubei Key Laboratory of Resource Science and Chemistry in Chinese Medicine,Hubei College of Traditional Chinese Medicine,Wuhan 430061,China  
AIM:To develop the chromatographic fingerprint of supercritical fluid CO 2 extract of Origanum vulgare L.by GC.METHOD:Supercritical fluid CO2 extraction was used to extract the essential oil from Origanum vulgare L..The chromatographic conditions were as follows:DB-17(30 m×0.25 mm,0.25 μm)capillary column;carrier gas N2,flow rate 1.5 mL·min-1,split ratio 201;column temperature program 80 ℃ lasting 2min,from 80 ℃ to 270 ℃ at rising rate 4 ℃/min and keeping 2min.RESULT:17 peaks were identified by GC-MS.The peak area of these components was over 90% of total area in the chromatographic fingerprint.RSD of precision and reproducibity was in the range of 5%.CONCLUSION:The method may provide reliable foundation of quality control of Origanum vulgare L..
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