A study on Hemagglutination reaction of Escherichia coli
Yang Zheng-shi, et al., National institute for the Control of Pharmaceutical and Biological Products, Beijing 100050
HA reaction of Escherichia coli from different sources to some kinds of RBC was reported by this paper. Satosfactory results were obtained performing in 4 C condition with Evans methods and phosphatebuffered agar. MRHA of E.coli from faeces of human to RBC of guinea pig occupied 27.3% and 2.7% for E.coli from urine. MRHA of E.coli from faeces of human and pig to RBC of A type occupied 0-4% and 41.3% for E.coli from urine. MRHA of E.coli from urind to RBC of P_2~K type were 41.3% and 12% to type. Corresponding rate of MRHA of E. coli from urine to A type and to P_2~K type was 97%. Fimbriae of bacteria adhesed to surface of A type RBC were showed by scanning electron microscope. A type of RBC can be in- stead of rare P_2~K to identify uropathogenic Escherichia Coli using HA.