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《CHINESE JOURNAL OF MICROECOLOGY》 2000-01
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FLUORESCENCE IN SITU PROBING OF BIFIDOBACTERIA

XiuShuli,Xiong Dexin,Yang Yi, et al. (Trauma Center Laboratory,the PLA 304 th hospital,Beijing,100037)  
Objective:To determine the value of fluorescence in situ hybridization (FISH) in identiying genus Bifidobacterium. Methods:Eight strains of Bifidobacterium as well as ten aerobic and anaerobic bacillus in logarrithmic growth phase were used.Hybridization was performed with Bifidobacterium genus-specific 16S rRNA oligonucleotide probe and universal kindom probe under same condition.Hybridization results were observed with OLYMPUS VANOX microscope.The fluorescence photograph and phase-contrast photograph of the same field were taken and hybridizaton percentage was calculated. Results: All Bifidobacteria hybridized with the two probes,their fluorescence contrasted obviousely agaist the background and hybridization percentage was more than80%. Whereas other strains only hybridized with universal-kindom probe. Conclusions: The selected oligonucleotide probe targeting 16S rRNA can be used to identify Bifidobacterium genus using in-situ hybridization methord.
【CateGory Index】: R372
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