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《Chinese Journal of Microecology》 2006-01
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Sequencing and characteristics analysis of 16S~23S rDNA ISR from oxyen-resistant Bifidobacterium

WU Yong-jun,LI Da-na,MENG Wang-ni,WANG Jia-fu(Life Science College,Guizhou University,Guiyang 550025,China)  
Objective Four types of rDNA 16S~23S intergenic spacer region(ISR) from oxygen-resistant Bifidobacterium were cloned and sequenced.Method Separated the PCR products from the agrose gel.Results The ISR sequence of Bifidobacterium longum containing 568 bp,was the same as Bifidobacterium breve and they were different from Bifidobacterium adolescentis and Bifidobacterium infantis which were only 510 bp.Through the ISR sequence analysis of 19 kinds Bifidobacterium and 4 kinds of oxygen-resistant Bifidobacterium,the results showed that there was a conservative and characteristic sequence of these genes,which could be used to the molecular marker of Bifidobacterium.Moreover,there were 3 high-variable regions,which could be provided a good basis for species identification work.Conclusion Clusters taxonomy analysis of 23 Bifidobacterium indicatied that the ISR sequence of the oxygen-resistant had already changed,it had laid a foundation for the further research of the evolution mechanism of Bifidobacterium under the aerobic condition.
【Fund】: 贵州大学科学基金资助
【CateGory Index】: R371
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 Wu Yongjun et al.;Study on Cell Growth and Metabolism of Oxygen-Resistant Bifidobacterium[J];Food Science;2001-10
2 DONG Xiu_Zhu, SHEN De_Long, XIN Yu_Hua Author’s address Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080;Phylogenetic relationship between the genus Bifidobacterium and related bacteria based on 16S rDNA homology[J];CHINESE BIODIVERSITY;2000-02
3 Dong Mingsheng et al. (Coll. Food Sci. & Tech. Nanjing Agric. Univ. Nanjing 210095);DNA FINGERPRINTING CHARACTERIZATION OF Bifidobacteria[J];Journal of Microbiology;2001-03
4 Zhou Hui School of Life Sciences,Zhongshan University,Guangzhou 510275;Cloning and Sequence Analysis of Two Types of rDNA 16 s ̄23 s Intergenic Spacer from Salmonella enteritidis[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI;1997-05
5 QU LianghuBiotechnology Research Center, Zhongshan University, Guangzhou 510275,China,CHEN Yueqin;Key to Molecular TaxonomyPrinciples and Methods[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Yu-fen1,ZHOU Xian-zhi1,CHEN Sheng1,CHEN Yang1,ZHAO Yi-jie2(1.Agricultural Bio-resources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian350003,China;2. Fuzhou Institute of Agricuhural Sciences,Fuzhou,Fujian 350018,China);Phylogeny of Cucurbita Based on ITS sequence data[J];Fujian Journal of Agricultural Sciences;2010-02
2 YAN Yan-hua1,JIN Hua2,HOU He-sheng1,WANG Ying3,JIANG Guo-bin2*(1.College of Life Sciences,Liaoning Normal University,Dalian,Liaoning 116029;2.College of Life Sciences,Dalian Nationalities University,Dalian,Liaoning 116600;3.College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110161,China);ITS Sequence Analysis of Stress-resistant and Fast-growing Populus wutunensis and Its Related Species[J];Guizhou Agricultural Sciences;2011-04
3 Mao Yunxiang, Yang Guanpin, Zhang Baohong, Zhang Xuecheng (College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003);Application of the Sequences Analysis of the 16S rRNA Gene and ITS of 16S-23S rRNA to the Systematic Study of the Genus Arthrospira and Spirulina[J];High Technology Letters;2001-06
4 ZHANG Jie,KANG Ji-chuan,WU Xing-liang(1.The Key Laboratory of Agri-biotechnology,Guizhou University,Guiyang 550025;2.Guizhou Academy of sciences,Guiyang 550001;3.The Colloge of Life Science,Shanxi Normal University,Linfen 041004);PRESENT STATUS OF MOLECULAR SYSTEMATICS FOR GENUS GANODERMA[J];Guizhou Science;2006-02
5 SHI Yang-qiang,ZHUANG Gui(College of Bioengineering,Henan University of Technology,Zhengzhou 450052,China);Development of Detection and Identification of Bifidobacteria[J];Modern Food Science and Technology;2007-05
6 MENG Jing-jing,HE Xue-li,LI Jun(College of Life Sciences,Hebei University,Baoding 071002,China);Application of molecular biological techniques on mycorrhizal research[J];Hebei Journal of Forestry and Orchard Research;2009-03
7 MA Fang,WEI Li,SHAN Dan,ZHOU Hao,KE Bao-qing(School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China);Identification and research of growth factor of one function bacteria of sulfate reducing bacteria——Anaerofilum pentosovorans A9[J];Journal of Harbin Institute of Technology;2007-02
8 WEI Li1,MA Fang1,CHEN Zhong-xi1,2,LIU Guang-min1,AOLEI Na-ri1(1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China, 2.Daqing Oil Field Design and Research Institute,Daqing 163712,China);The isolation and the phylogenetic analysis of Hydrolyzed Polyacrylamide(HPAM) Degraded Bacteria[J];Journal of Harbin Institute of Technology;2007-08
9 ZHAO Li-jun1,2,WEI Li1,MA Fang1,LI Fen1,ZHANG Jian-qi3 (1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2. School of Chemistry Science and Engineering,China University of Petroleum-Beijing,Beijing 102249,China; 3.Department of Civil and Environmental Engineering,University of Maryland,Baltimore County,Baltimore,MD,USA,21250);Phylogenetic analysis of sulfate reducing bacteria based on terminal products and sequence genes[J];Journal of Harbin Institute of Technology;2008-12
10 XIE Xiuping~ 1 ,YAN Yanchun~ 1,2, ,LIU Pingping~ 1 ,WANG Shenghui~ 2 1.State Key Laboratory of Crop Biology, College of Life Science of Shandong Agricultural University, Tai'an, 271018 2. Graduate School of Chinese Academy of Agricnltural & Science, Beijing 100081;Isolation,degradation and characterization of methylparathion degradative strain L4[J];Acta Scientiae Circumstantiae;2006-10
China Proceedings of conference Full-text Database 3 Hits
1 LI Hai-lei SUN Hong-chun ZHANG Qi-zhi WANG Wen-liang SUN Shou-yi WANG Shou-jing YAN An-quan LI Zhi-guo (Institute for Application of Atomic Energy,Shandong Academy of Agricultural Science,Jinan,Shandong 250100,China);Isolation and degradability study of methylparathion degrading strain L-W[A];[C];2007
2 Liao Fupin Chen Haiying Lin Jianrong Song Jiaqing Huang Xuhua Li Wenchu Feng Qi (College of Animal science.South China Agricultural University,Wushan.Guangzhou.510642.China);Identification of a producing antibacterial protein CP7 strain and researches of its antibiotic activity[A];[C];2004
3 GAO Jinguo,TIAN Xingjun,CHEN Bin,ZHU Jing,YANG Changlin, LI Zhongqi and SONG Fuqiang (School of Life Science,Nanjing University,Nanjing 210093);Application of DNA Molecular Markers in Plant Phylogeny Study[A];[C];2002
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Wu Yongjun;The antergia of intertind flora and biodegranation of enterotoxigenorig were studied by oxygen-resistant bifidobacterium[J];FOOD SCIENCE;1999-12
2 Wang Zhanwu(Insitute of Agro-Physics,Physiology and Biochemistry, Hebei Academy of Agriculture and Forestry,Shijiazhuang 050051)Zhang Chi(Department of Food Science, China Agriculture University, Beijing 100094);EFFECTS OF DIFFERENT ACIDIC CONDITIONS ON THE SURVIVAL OF BIFIDOBACTERIA[J];MICROBIOLOGY;1997-02
3 Yang Jichu;Liu Jia(Department of Chemical Engineering,Tsinghua University,Beijing,100084);STUDY OF CELL GROWTH AND METABOLISM OF BIFIDOBACTERIUM[J];MICROBIOLOGY;1995-06
4 Wu Yongjun Wang Jiafu Yin Junfeng (Guizhou Agricaltural College Guiyang, 550025 CHINA);ISOLATION AND IDENTIFICATION OF BIFIDOBACTERIA[J];SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES;1997-03
5 Zhou Hui School of Life Sciences,Zhongshan University,Guangzhou 510275;Cloning and Sequence Analysis of Two Types of rDNA 16 s ̄23 s Intergenic Spacer from Salmonella enteritidis[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI;1997-05
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