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《Chinese Journal of Microecology》 2006-01
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Isolation and identification of an antimicrobial endophytic strain EJH-2

SUN Li-jun~(1,2),LU Zhao-xin~2,BIE Xiao-mei~2,SUN De-kun,YANG Sheng-yuan~2(1.School of Industry,Anhui Science and Technology College,Fengyang 233100,China;2.School of Food Science,Nanjing Agricultural University,Nanjing 210095,China)  
Objective One endophytic strain EJH-2 with strong antimicrobial activity was derived from tissue of Lonicera japonica.Methods Thunb and identified based on 16S rRNA gene sequences.The sequence fragment of 1 300 bp 16S ribosomal RNA was amplified from total DNA of strain EJH-2 by PCR.The sequence analysis was down by the application of BLAST on the websites of NCBI and the phylogenetic tree was drawn by Bioedit 7.0 and Treedrawing.Results EJH-2 shared 99.76% 16S rRNA sequence homology with Paenibacillus polymyxa KCTC 1663.EJH-2 and Paenibacillus polymyxa were in same branch of phylogenetic trees.Conclusion The strain EJH-2 belongs to Paenibacillus polymyxa.
【Fund】: 安徽科技学院重点学科建设基金资助(YZD2004-16)
【CateGory Index】: Q93-331
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