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《Chinese Journal of Microecology》 2006-01
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The cloning of the mouse BALB/c metallothionein-1 cDNA in the plasmid expression vector pNZ2103 of Lactococcus

LIN Lin,REN Feng,ZHAO Bao-chang(Dalian Medical University,Dalian 116027,China)  
Objective To clone the mouse BALB/c metallothionein-1(MT-1)cDNA in the plasmid expression vector pNZ2103 of Lactococcus.Methods Two primers were designed with the digestion site of Pst 1 and of Hind Ⅲ.With the plasmid pET21a-MT used as the template,MT-1 cDNA was amplified by PCR.MT-1 cDNA was cloned into pNZ2103 with the restriction endonuclease Pst Ⅰ and Hind Ⅲ to form the recombinant plasmid pNZ2103-MT.pNZ2103-MT was transformed into the strain DH5α.The transformant containing the plasmid pNZ2103-MT was identified by PCR and digestion of Pst I and Hind Ⅲ.The metallothionein expression capacity of pNZ2103-MT in DH5α was identified on 12% SDS-PAGE.Results The transformant(DH5α) containing the plasmid pNZ2103-MT was obtained.Conclusion MT expression of pNZ2103-MT in DH5α was identified on 12% SDS-PAGE.It was difficult to decide MT expression level because the amount was too low.
【Fund】: 辽宁省教委资助(990221059)
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIN Lin,DING Wen yong,REN Feng,ZHAO Bao chang (Dalian Medical University,Dalian 116027,China);PURIFICATION OF THE MOUSE BALB/C METALLOTHIONEIN-1 FUSION PROTEIN[J];Chinese Journal of Microecology;2003-01
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 CHENG Yu-liang,YAO Wei-rong,QIAN He,YANG Mei-lin (Food Department,Southern Yangtze University,Wuxi 214036,China);THE STUDY OF MUTATION AND BREEDING OF THE STRAINS PRODUCING MT-LIKE[J];Food Research and Development;2006-02
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 XU Bo1, CAO Yu-sheng1*, CHEN Yan1, GUO Xing-hua2(1.The Key Laboratory of Food Science of MOE, Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047; 2.Institute of Microbiology, Chinese Academy of Science, Beijing 100083);Construction of NICE system food-grade vector prnb48 for lactococcus lactis[J];Food Science and Technology;2006-10
2 YAO Xiao-ying,LI Da-jin~*,YUAN Min-minLaboratory for Reproductive Immunology,Hospital and Institute of Obstetrics & Gynecology,Shanghai Medical College,Fudan University,Shanghai 200011;The expression of fusion protein hCGβ-C3d3 in Lactobacillus[J];Journal of Reproductive Medicine;2006-05
3 CHEN Si-wei~ 1,3 ,ZHONG Jin~ 1 ,HUAN Lian-dong~2( ~1 State Key Laboratory of Microbial Resources, ~2 Center for Microbial Metabolism and Metabolic Engineering,Chinese Academy of Sciences,Beijing 100101,China)( ~3 Graduate School of the Chinese Academy of Sciences,Beijing 100039,China);Expression of human insulin in lactic acid bacteria and its oral administration in non-obese diabetic mice[J];Acta Microbiologica Sinica;2007-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LIN Lin, REN Feng, ZHAO Bao-chang, JIN Xiao-yan (Dalian Medical University, Dalian 116027);Efficient Expression of the Mouse Balb/C Metallothionein-1 Gene in Escherichia Coli[J];Pharmaceutical Biotechnology;2001-03
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