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《Chinese Journal of Microecology》 2006-01
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Establishment and application of a rapid precise method to detect Staphylococcus aureus enterotoxins A

ZHANG Hong-he,ZHANG Wei-ying,DONG Xian-qin,LU Zhong,WANG Xiao-jun,CHEN Yu(Diagnostic Laboratory,the First People's Hospital of Hangzhou,Hangzhou 310006,China)  
Objective To establish a rapid specific quantitative assay for the detection of Staphylococcus aureus enterotoxins A.Methods Design and synthesize the primers and TaqMan probe targeted at the genes of femB and SEA.68 isolates of Staphylococcus aureus originating from stool smaples were analyzed.Results The sensitivity of the assay for Staphylococcus aureus enterotoxins A was 1.0×10~2 copies.Among 68 samples,11 strains were positive for enterotoxins A(16.2%,11/68).Conclusion The TaqMan PCR could rapidly exactly detect the Staphylococcus aureus enterotoxins A.
【CateGory Index】: R450
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