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《Chinese Journal of Microecology》 2008-01
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The effects of Chinese herb medicine compound on the intestinal microflora and blood visible compositions of Grass carp

ZU Guo-zhang1,LI Jin-nian1,ZHANG Chuan-liang2,ZHANG Xiao-hua1,WEI Zhong1,XU Jian-xin3(1.Animal Science and Technology College of Anhui Agricultural University,Hefei 230036,China;2.Agricultural Commission of Changxing County,Changxing 313104,China;3.Science College of Anhui Agricultural University,Hefei 230036,China)  
Objective To explore the effects of Chinese herb medicine compound on the intestinal microflora and blood visible compositions of Grass carp.Methods Grass carp was selected as an animal model,the intestinal microflora number and dominant flora of Grass carp were detected by plate pouring method and ATB semi-auto bacteria identification method respectively before and after feeding on Chinese herb medicine compound.Meanwhile,the number of red and white blood cells and the content of hemaglobin were measured by routine blood cell counting method and improved Shalishi's method respectively.Results The total intestinal bacteria of Grass carp of the control group was 6.6×108 CFU/g,and the dominant flora included Aeromonas,Pseudomonas and Enterobacter;After 10 d feeding on the concentration of 2.5%,5.0% and 7.5% Chinese herb medicine compound,the total intestinal bacteria reduced to 3.0×107 CFU/g,5.0×106 CFU/g and 1.5×107 CFU/g respectively,and the dominant flora changed to F.indologenes,P.alcaligenes and X.campestris.The number of white blood cells and the content of hemaglobin in treatment group was significantly higher than that of control group respectively,but no significant variation was found in the number of red blood cells between control and treatment group.[WTHZ]Conclusions The Chinese herb medicine compound composed of Rhizoma coptidis,Rheum officinale,Flos lonicerae,Fructus forsythiae,Radix astragali and Glycyrrhiza uralensis had some effects on intestinal microflora and blood compositions of Grass carp,and this compound had better immune and antiinfection function.
【Fund】: 省自然基金项目070411019;; 省教育厅项目99JL0048 KJ2007A100 2006KJ170B
【CateGory Index】: S948
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