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《Chinese Journal of Microecology》 2008-01
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A comparison of three protocols for the extraction of total fecal DNA

SHEN Jian,ZHANG Bao-rang,WEI Hua,PANG Xiao-yan,ZHAO Li-ping(School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)  
Objective To investigate the effects of different fecal DNA extraction methods on the molecular analysis of the diversity of gut microflora.Methods Three protocols-bead beating,chemical lysis method and QIAamp DNA Stool Mini Kit were used to extract total DNA from one fecal sample of an adult.The DNA yield and the DGGE profiles of the amplicons of 16S rRNA gene V3 region of the three DNA isolation protocols were compared.Results The DNA yield of bead beating was 2 times higher than that of the other two protocols.The Dice coefficient between DGGE profiles of these three protocols was from 60%~70%.Two predominant bands only appeared in the DGGE patterns obtained with the bead beating method.The DNA isolation experiments using 2~5 min duration of bead beating showed that DNA yield slightly increased with the increasing beating time but the DGGE patterns remained constant.Conclusion Different DNA isolation methods can affect the diversity analysis of the microbiota due to their different lysing efficiency,bead beating is more appropriate for DNA extraction from fecal samples because it can give higher DNA yield and recover more diversity.
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 3 Hits
1 LONG Wen-Min~ 1 , YAN Er-Ping~1, XUAN Tao~1, WEI Hua~2, PANG Xiao-Yan~2, ZHAO Li-Ping~2, MA En-Bo~ 1 (1.Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; 2.School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China);Comparative studies on the methods of bacterial genomic DNA extraction from grasshopper guts[J];Chinese Bulletin of Entomology;2009-03
2 DU Cai-he,ZHOU Dong-rui,XIAO Fei,ZHANG Ren-min,WEI Ting-ting,HU Fang(Learning & Science Research Centre,Southeast University,Nanjing 210096,Jiangsu);Screening of Intestinal Microflora Diversity[J];Journal of Nanjing Xiaozhuang University;2010-03
3 ZHANG Yi1,ZHANG Chen-hong1,SHEN Jian1,LI Hou-kai2,NI Yan2,ZHANG Xiao-jun1,ZHAO Li-ping1,PANG Xiao-yan1(1.Lab of Molecular Microbial Ecology and Ecogenomics,School of Life Science and Technology,Shanghai Jiaotong University,Shanghai 200240,China;2.School of Pharmacy,Shanghai Jiaotong University,Shanghai 200240,China);Intestinal microbiota structure of diet-induced obesity rats and its specific molecular marker[J];Chinese Journal of Microecology;2009-04
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Jian-hua, CHEN Zhang-liu, LI Yun, YU Dao-jin (Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation/College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642,China);Development of denaturant gradient gel electrophoresis for analysis of intestinal microflora community diversity[J];Chinese Journal of Veterinary Science and Technology;2005-06
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Wu Yinbao~1 Shi Jincai~1 Mo Cehui~(2) Lan Xiaohua~1 Chen Wenyan~1 1.College of Animal Science,South China Agricultural University,Guangzhou 510642,China; 2.College of Science and Engineering,Jinan University,Guangzhou 510632,China;Comparison of methods for microbial DNA extraction from swine feces and soil[J];Transactions of the Chinese Society of Agricultural Engineering;2006-S2
2 XU Wen-tao1,2,GUO Xing1,LUO Yun-bo1,HUANG Kun-lun1,2 (1.Laboratory of Safety Detection and Risk Assessment, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Supervision, Inspection and Testing Center of Genetically Modified Orginisms, Ministry of Agriculture, Beijing 100083, China);Research Progress on Analysis Methods of Diversity of Microbial Flora[J];Food Science;2009-07
3 JIN TING-ting1,2,WANG XIU-li2,WANG Xiao-mei1(1.Tianjin Key Laboratory of Aqua-Ecology & Aquaculture,Department of Fisheries Science,Tianjin Agricultural University,Tianjin 300384,China;2.Dalian Fishery University,Dalian 116023,Liaoning Province,China);Study on Parameters of PCR and Electrophoresis in DGGE Technique on Analyzing Diversity of Mitochondrial Cytochrome b Gene of Eriocheir sinensis[J];Journal of Tianjin Agricultural University;2008-02
4 QIN Yu-ling1,2,ZHAO Jing-jing1,HUANG Xian-hui1,GAO Yan-ling1,CHEN Zhang-liu1,ZENG Zhen-ling1,LIU Jian-hua1(1College of Veterinary Medicine,National Reference Laboratory of Veterinary Drug Residues,South China Agricultural University(SCAU),Guangzhou 510642;2Guangdong Institute of Veterinary Drug and Feedstuffs Control,Guangzhou 510220);Effects of Enrofloxacin on Human Intestinal Mocrofloa in Chemostat Model[J];Scientia Agricultura Sinica;2009-12
China Proceedings of conference Full-text Database 1 Hits
1 WU Yin-Bao, Shi JingCai, Lan XiaoHua, Chen WengYan (College of Animal Science, South China Agricultural University, Guangzhou 510642, China);Comparison of Methods for DNA Extraction from pig Feces and Soil Microorganism[A];[C];2006
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Fa-Jun 1, 2, ZHANG Jue-Feng1, XIA Zhan-En1, Lü Zhong-Xian1, YU Xiao-Ping11.Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China2.Agricultural and Biotechnological Colleges, Zhejiang University, Hangzhou 310029, China;MORPHOLOGICAL OBSERVATION ON THE YEAST-LIKE ENDOSYMBIOTES IN BROWN PLANTHOPPER, NILAPARVATA LUGENS[J];Acta Zootaxonomica Sinica;2006-01
2 TANG Li1 LIAN Jie2 LUXiaojun1 DAI Yurong2 TAN Xiaoshan2 ZHENG Changge1(1.Guangzhou Biological Control Station,Guangzhou,510460,2.College of life sciences Zhongshan University,Guangzhou,510275);ANTIFUNGAL ACTIVITY OF INTESTINAL BACTERIA OFBLATTELLA GERMANICA[J];Natural Enemies of Insects;2005-03
3 LIU,Y.S.;LEO,P,L.Institute of Entomology,Academia Sinica;ON THE ANATOMY AND HISTOLOGY OF THE DIGESTIVE SYSTEM OF THE ASIATIC MIGRATORY LOCUST,LOCUSTA MIGRATORIA MENILENSIS MEYEN[J];Acta Entomologica Sinica;1955-03
4 LU Zhong\|xian, YU Xiao\|ping, CHEN Jian\|ming, ZHENG Xu\|song, XU Hong\|xing, TAO Lin\|yong (Institute of Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China);Role of endosymbiote in virulence change of the brown planthopper to rice resistant varieties[J];Acta Entomologica Sinica;2001-02
5 XIANG Hui~1, LI Mu-Wang~ 1,3 , ZHAO Yong~2, ZHAO Li-Ping~2, ZHANG Yue-Hua~3, HUANG Yong-Ping~ 1, ?(1. Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 2. Department of Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; 3. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu 212018, China);Bacterial community in midguts of the silkworm larvae estimated by PCR/DGGE and 16S rDNA gene library analysis[J];Acta Entomologica Sinica;2007-03
6 ZHANG Xiao-Min, LI Xiao-Ling, GUO Ya-Ping, MA En-Bo ** (College of Life Science and Technology, Shanxi University, Taiyuan 030006, China);A new method for studying structure of alimentary canal in grasshopper.[J];Chinese Bulletin of Entomology;2007-01
7 XIANG Hui,HUANG Yong-Ping (Shanghai Institute of Plant Physiology and Ecology,Chinese Acadamy of Sciences,Shanghai 200032,China);Symbiosis between gut microbiota and insects[J];Chinese Bulletin of Entomology;2008-05
8 YANG Hong,PENG Jian-xin,LIU Kai-yu,HONG Hua-zhu~*(Key Laboratory of Pesticide & Chemical Biology,Ministry of Education;Institute of Entomology,College of Life Sciences,Central China Normal University,Wuhan 430079,China);Diversity and function of symbiotic microbes in the gut of lower termites[J];Acta Microbiologica Sinica;2006-03
9 LU Hai feng,WEI Gui fang,LI Zhong kui, et al . (School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China);ERIC-PCR based fingerprinting and molecular hybridization to analyze the characteristics of intestinal microflora of giant panda[J];Chinese Journal of Microecology;2005-02
10 LIU Yu-sheng1,LI Ming-li2,LIU Jun-zhan3,ZHENG Ji-fa1(1.Institute of Environmental Biology,College of Plant Protection,Shandong Agricultural University,Taian 271018,China;2.The General Plant Protection Station of Shandong Province,Jinan 25010,China;3.Binzhou Plant Protection,Binzhou 256618,China);Study on the intestinal bacteria in L.migratoria manilensis[J];Chinese Journal of Microecology;2007-01
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