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《Chinese Journal of Microecology》 2008-06
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Studies on extraction of bifidobacterium plasmid by mutanolysin from Streptomyces globisporus fermentation fluid

LUO Yao-ling,HUANG Xue-ping,WU Xiang-mei,DING Song-tao,MA Yong-ping,SONG fang-zhou(Department of Biochemistry and Molecular Biology,Chongqing Medical University,Chongqing 400016,China)  
Objective To find an economic method to extract the plasmid from Bifidobacterium.Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.Conclusion Mutanolysin from Streptomyces globisporus is an economic strategy to the plasmid extraction for Bifidobacterium.
【Fund】: 国家自然科学基金资助项目(30671865)
【CateGory Index】: Q93
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