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Screening characteristic proteins of Mycobacterium

HU Qi1,YANG Yong-chang2,HUANG Wen-fang2,LIU Hua2,YU Hua2,XIAO Dai-wen2,HUANG Bo1,FENG Wen-li1(1.Key Laboratory of Laboratory Medical Diagnostics(Designated by the Ministry of Education),Laboratory Medicine Department,Chongqing Medical University,Chongqing 400016,China;2.Clinical Laboratory Department,Sichuan Academy of Medical Science & Sichuan Provincial People′s Hospital,Chengdu 610072,China)  
Objective To screen characteristic proteins of Mycobacterium which may lay a foundation for its rapid identification.Methods Proteins of standard strains of Mycobacterium were extracted and detected by dry chemistry method.The proteins were analyzed by surface enhanced laser desorption/ionization time-of-flight mass spectra(SELDI-TOF-MS),compared with other bacteria,and the data was collected by Ciphergen Protein Chip software.The proteins of every stain were analyzed by SELDI for twenty times to evaluate the reproducibility of protein molecular mass.Results About twenty proteins were found in M.smegmatis 14468,and about forty proteins were found in M.tuberculosis ATCC 25177,M.terrae ATCC 15755,M.intracellulare ATCC 13950 and M.smegmatis ATCC 607.Compared with other bacteria,four proteins were only found in Mycobacterium.The CV of protein in molecular weight was less than 0.05% in Mycobacterium.Conclusion The Mycobacterium has characteristic proteins,which may lay a foundation for its rapid identification.
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