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《Chinese Journal of Microecology》 2008-06
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Construction and identification of human ribonuclease gene recombinant adenovirus

LIU Peng,ZHAO Bao-chang,FAN Jian-hui,TIAN Yu-xiang,YANG Fan,CUI Xiu-yun(Department of Biochemistry & Molecular Biology,Dalian Medical University,Dalian 116044,China)  
Objective To construct the recombinant adenovirus vector containing human RI for future gene therapy.Methods The RI fragment was amplified by PCR according to the pT7-RI,then the fragment was cloned to T vector and subcloned to pAdTrack-CMV.The linearized shuttle plasmid was homogenously recombined with pAdEasy-1 in BJ5183.The candidate clone was further analyzed by restriction endonuclease digestion and PCR.Then the recombined adenovirus was transfected into 293 cells for packaging and amplifying.The titer and its infection rate were determined using the green fluorescent protein(GFP) expression in the shuttle plasmid.Results Restriction endonuclease and PCR analysis confirmed that the human RI gene was successfully inserted into the adenovirus vector.The titer of the recombinant adenovirus was 1.5×1010pfu/ml.Conclusion The recombinant adenovirus containing human RI gene was successfully constructed by the method of homogenous recombination in bacteria.
【CateGory Index】: Q78
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【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 XIA Jun, YAN Ping, CHEN Jun-xia, et alDepartment of Biochemistry and Molecular Biology, DalianMedical University, Dalian 116027, China; Department of Biochemistry and Molecular Biology, Bengbu MedicalCollege, Bengbu 233003, China; Center Laboratory, Dalian Medical University, Dalian 116027, China);Protection Effects of Ribonuclease Inhibitor on the Cells Peroxidative Damage Injured by H_2O_2[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-01
2 TANG Xiao-yan1,ZHANG Ju1,XIA Jun2,CHEN Jun-xia3,CUI Xiu-yun4(1.Research Center of Clinical Laboratory Science,2.Department of Biochemistry and Molecular,Bengbu Medical College,Bengbu Anhui 233030;3.Department of Biochemistry and Molecular,Chongqing Medical University,Chongqing 400016;4.Department of Biochemistry and Molecular,Dalian Medical University,Dalian Liaoning 116027,China);The relevant research between expression of ribonuclease inhibitor and the malignant proliferation in human breast cancer cells[J];Journal of Bengbu Medical College;2008-06
3 TANG Xiao-yan1,XIA Jun2,ZHANG Ke-chang1,CHEN Jun-xia3,CUI Xiu-yun4(1.Research Center of Clinical Laboratory Science,2.Department of Biochemistry and Molecular Biology,Bengbu Medical College,Bengbu Anhui 233030;3.Department of Biology,Chongqing Medical University,Chongqing 400016;4.Department of Biochemistry and Molecular Biology,Dalian Medical University,Dalian Liaoning 116027,China);Inhibitory effect of transfection of human ribonuclease inhibitor on the proliferation in breast cancer cells[J];Journal of Bengbu Medical College;2009-01
4 FAN Jian-hui,LIU Peng,CUI Xiu-yun,TIAN Yu-xiang(Department of Biochemistry and Molecule Biology,Dalian Medical University,Dalian 116044,China);Establishment and identification of breast cancer cell line with over-expressed human ribonuclease inhibitor[J];Chinese Journal of Microecology;2008-03
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 LI Yin-lan,LI Jing-min.(Department of Ophthalmology,The Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China);Advancement of Ribonuclease Inhibitor[J];Medical Recapitulate;2007-05
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