Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Microecology》 2008-06
Add to Favorite Get Latest Update

Cloning and analysis of the mutational core gene of the hepatitis B virus

ZHANG Hong-qin,LI Pei-zhen,YING Jun(Central Laboratory of Biology of Wenzhou Medical College,Wenzhou 325027,China)  
Objective To clone and analyze the deleted form of the hepatitis B virus core gene.Methods The deleted form of hepatitis B virus core gene was obtained by PCR from a hepatitis B virus genome.The PCR product was ligated into pUCm-T vector and sequenced.Result The cloned segment was 454 bp with 98 bp between 220 bp and 317 bp being lost.Conclusion The deleted form of hepatitis B virus core gene was cloned successfully which could be used for expression and function researches.
【CateGory Index】: Q786
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 WANG Yongfu,GUO Chunlin,GENG Lixia,et al (The First Affiliated Hospital,Inner Mongolia University of Science and Technology,Baotou 014010,China);The Relationship between Hepatitis B Virus Core Mutant and Effect of Interferon[J];Journal of Baotou Medical College;2003-04
2 ZHANG Ji ming, LIU Fang, YIN You kuan, et al. Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China;The cloning and comparative study of full-length HBV genomes from serums before and after treatment with lamivudine in a patient with hepatitis B[J];Chinese Journal of Infectious Diseases;2003-04
Chinese Journal Full-text Database 6 Hits
1 LI Lei,LI Yi,GAO Rentao,et al.Department of Infectious Disease,Anhui Provincial Hospital,Anhui Medical University,Hefei 230001,China;The biological changes of hepatitis B virus in a patient with acute-on-chronic liver failure induced by lamivudine-resistant mutant infection[J];Journal of Clinical Hepatology;2011-01
2 GU Qiang-ye, CHEN He-min.(Jining Municipal Infectious Disease Hospital, Jining, Shandong 272031);Therapeutic efficacy of combined application of Lamivudine and Ganluoxinpills in treating chronic hepatitis B and its in fluence on YMDD motif[J];Chinese Journal of Clinical Hepatology;2005-02
3 GU Qiang-ye,LI Qin,GE Xiu-lin(Jining City Hospital for Infectious Diseases,Jining,Shandong 272031,China);Observation of long-term efficacy of different treatment protocol of Adefovir dipivoxil in chronic hepatitis B patients with Lamivudine-resistance[J];Journal of Clinical Hepatology;2011-04
4 Hu Xiaoping1,Zhao Zhongfu2 (1.Changzhi medical school,Changzhi Shanxi 046000;2.Changzhi medical college,Changzhi Shanxi 046000);Effect of Kushenin Combined with Lamivudine in Treating Chronic Hepatitis B and its Influence on YMDD Motif Mutation[J];Journal of Shanxi College of Traditional Chinese Medicine;2009-06
5 HUANG Xin-zhou (Department of infectious Disease,The people's hospital of Chibi,Chibi 437300);Therapeutic efficacy of combined application of Lamivudine and Oxymatrine in treating chronic hepatitis B and its influence on YMDD motif[J];China Medical Herald;2007-11
6 Nan Yu,Jin Cui,Guo-Bao Zhou,Yun-Jiao Zhang,Jing-Li Chen,Central Biological Laboratory,Department of Infectious diseases,Guangzhou Development District Hospital,Guangzhou 510730,Guangdong Province,China;Variations in the large surface protein gene of hepatitis B virus in vivo:a longitudinal study and application[J];World Chinese Journal of Digestology;2009-27
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved