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《Chinese Journal of Microecology》 2009-01
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Effects of allitride on adhesion and exopolysaccharide of Pseudomonas aeruginosa biofilms

LIN Li-hua,YU Jia-lin,LIN Ya-yin,LIU Guan-xin(Children's Hospital of Chongqing Medical University,Chongqing 400014,China)  
Objective To investigate the effects of allitride on adhesion of Pseudomonas aeruginosa biofilm at early stage and Extracellular Polymeric Substances(EPS).Methods Multifunction fluorometer was used to measure fluorescence of green fluorescent protein(GFP)in pGFPuv transformated PA01 in 96-well plate.The adhesion ratio of PA01 was calculated to determine the effects of allitride on PA01 biofilm adhesion,and qualitative study adherence of GFP-PA01 was performed by fluorescence microscope.The fluorescein isothiocyanate(FITC)conjugated concanavalin A(FITC-conA)was used to stain the EPS,in order for the qualitation of EPS by fluorescence microscope;the phenol-sulfuric acid method was used to quantitate the EPS in each group.Results Six-hour group:after intervention with high dose of allitride,the adhesion ratio was decreased from 0.70±0.03 to 0.50±0.01,t=15.014,P0.05.Low dose of allitride could also affect the adhesion ratio,but not so obvious as high dose.Other time groups except the 9-hour group had an approximate tendency as the 6-hour group,which may be related with unstability of allitride.Compared with normal saline control group,the colony distribution of GFP-PA01 became rarefactive after intervention,especially with high doses.The EPS in biofilm was significantly decreased after intervention with allitride,which was visualized by fluorescence microscope,based on the FITC-conA staining.Quantification of EPS showed that total EPS decreased from(602.66±21.94)μg to(181.19±1.59)μg after high dose of allitride intervention(t=60.589,P0.05).Conclusions Allitride could decrease the adhesion and EPS producing ability of PA01 significantly.
【Fund】: 国家自然科学基金(30772363);; 重庆市自然科学基金(渝科发计字2007-340)
【CateGory Index】: R285.5
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