Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Microecology》 2009-01
Add to Favorite Get Latest Update

Effect of lipoteichoic acid of Bifidobacterium on NKG2D receptor and its ligands of NK cell in mouse bearing B16 cancer

LI Fu-rong,WANG Yue,LIU Ming-fang,XIE Ning,WANG Qiang(Key Laboratory of Laboratory Medical Diagnostics of Education Ministry,Department of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China)  
Objective To explore the effect of lipoteichoic acid(LTA)of Bifidobacterium on NKG2D receptor and its ligands of NK cell in mice bearing melanoma B16 cancer.Methods Melanoma B16 cells were subcutaneously injected into C57BL/6 mice;LTA of Bifidobacterium was injected subcutaneously into the mice when the tumor was palpable.The changes of the killing activity of NK cells were detected by MTT assay;the expression of NKG2D protein in splenic NK cell and the mRNA expression of Rae-1 and H60 in tumor tissue were detected by flow cytometry(FCM)and RT-PCR respectively.Results Compared with the control group,the killing activity of NK cell,the expression of NKG2D protein in NK cells and Rae-1,H60 mRNA expression in the tumour tissue were enhanced(P0.05)in dose-dependent manner after treatment with different dose of LTA of Bifidobacterium.Conclusions LTA of Bifidobacterium can enhance the killing activity of NK cells in mice bearing melanoma B16 cancer.The mechanism is possibly correlated to the up-regulated expression of NKG2D protein in NK cells and the mRNA expressions of Rae-1 and H60 in tumor tissue.
【CateGory Index】: R371;R73-3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved