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《Chinese Journal of Microecology》 2009-10
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Study on the change of Th1/Th2 cytokine after extraintestinal rotavirus infection in suckling mice

XIA Qun,CHEN Lan-ju,ZHOU Rui,CHEN Ming-wu,DING Zhou-zhi(Department of Pediatrics,the First Affiliated Hospital,Bengbu Medical College,Bengbu 233003,China)  
Objective To assess the distribution characteristics of Th2-type cytokine(interferon-γ,IFN-γ)and Th2-type cytokine(interleukin-10,IL-10)in the serum of ICR suckling mice inoculated by intraperitoneal injection with Simian Rotavirus SA11,so as to gain insight into the possible role of the systemic Th1/Th2 cell-mediated immune response in the pathogenesis of extraintestinal rotavirus infection.Method 48 ICR suckling mice were randomly divided into three groups:extraintestinal groups inoculated by intraperitoneal injection with Simian Rotavirus SA11,intestinal groups inoculated by oral gavage with tales doses of rotavirus,and control groups with no special handling.At 4 and 8 days after infection,the pathological changes of extraintestinal tissue were examined under light microscopy and the levels of IFN-γ and IL-10 in the serum were measured with enzyme linked immunosorbent assay(ELISA).Result Varying degrees of pathological changes were observed in the liver,kidneys,spleens and lungs in the extraintestinal group.4 days after infection,the levels of IFN-γ began to increase in extraintestinal and intestinal groups,then decrease at the days 8 to the level of base line.IL-10 in extraintestinal group increased on day 4,then decreased on day 8,yet still increasing compared with the control group.In intestinal groups,the IL-10 had no significant change.Conclusion After extraintestinal rotavirus infection,the cell immune respone showed Th1-Th2 type immune response in the early stage.But in the late stage,IL-10 expression predominated and the cell immune respone switched to Th2 type immune response.Th1/Th2 imbalance in cytokines may be an important factor in extraintestinal rotavirus infection.
【Fund】: 安徽省教育厅自然科学研究项目(2006KJ107C)
【CateGory Index】: R725.1
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