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《Chinese Journal of Microecology》 2009-10
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The influence of whole peptidoglycan of Bifidobacterium on interleukin-12 produced by dendritic cells derived from bone marrow of mice in vitro

ZHAO Li-li,CHENG Qian(Children's Hospital of Chongqing Medical University,Chongqing 400014,China)  
Objective To explore in vitro the effect of whole peptidoglycan of Bifidobacterium(WPG)in a same dose on the interleukin 12 secretion by the DCs deriving from bone marrow at different time.Method In vitro,immature DCs were induced by monocytes cultured in granulocytes macrophage-colony stimulating factor(GM-CSF)and interleukin-4 on the 7th day and then exposed to WPG(5 μg/ml).The supernatant was collected at 0 hour,12 hour,24 hour,36 hour,48 hour and 60 hour respectively after exposing to WPG,and the level of interleukin 12 was detected by Enzyme-labeled immunosorbent assay(ELISA).Result The level of interleukin-12 secreted by dendritic cells co-cultured with WPG increased gradually in 24 hours,reached the peak at 24 hour and then decreased 24 hours later.Compared with the 0 hour group,the levels of IL-12 in the 12 hour group,the 24 hour group and the 36 hour group were significantly higher(P 0.05),while neither the 48 hour group nor the 60 hour group were significantly different from the 0 hour group(P 0.05).Conclusion The level of IL-12 secreted by DCs derived from bone marrow had been remarkably elevated under the stimulation of WPG.Meanwhile the immunostimulation of WPG had time-effect relationship.
【Fund】: 重庆市教委项目(KJ090312)
【CateGory Index】: R371
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