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《Chinese Journal of Microecology》 2009-10
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Purification of natural mutacin in liquid medium

LI Song 1,YANG Liu 1,XU Xiao-fang 1,YANG Bai-xia 2,LIU Wen 1(1.College of Stomatology,Anhui Medical University,Hefei 230032,China;2.Department of Clinical Laboratory,the First Affiliated Hospital of Anhui Medical University,Hefei 230022,China)  
Objective To separate and purify mutacin produced from liquid medium by Streptococci mutans(S.mutans)strains,and lay a foundation for further molecular biological research of mutacin.Method The antibacteria activity of 80 strains of S.mutans were tested by the stab culture technique against Streptococcus oralis ATCC 10557.The mutacin produced by the strain "1G" was initially purified by SPE(Solid-phase extraction)after crude extraction by chloroform.And then the active substances were purified by RP-HPLC(Reversed-phase high performance liquid chromatography)twice.Then the purified target peptide(mutacin)was collected and freeze-dried for further study.Result Roughly 15 μg of crude mutacin was extracted from 200 ml of liquid medium of the strain "1G".Then the purified mutacin was obtained through SPE and twice RP-HPLC.Conclusion The separation and purification of mutacin has been a complex procedure due to its small molecular mass.This study on the purification of mutacin may contribute to further research of mutacin.
【Fund】: 国家自然科学基金(30640074);; 安徽省教育厅重点项目基金(KJ2007A027);; 安徽医科大学博士基金(2004)资助
【CateGory Index】: R378
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 Ding Yun, Ling Junqi, Chen Han. Guanghua College of Stomatology,Sun Yat-sen University, Guangzhou 510055;A study of peptide vaccine HDS from Streptococcus mutans glucosyltransferase——the synthesis and appraisement of peptide HDS[J];Journal of Practical Stomatology;2003-04
2 Shi Xin,Ni Longxing, Tian Yu,et al.Department of Conservative Dentistry,College of Stomatology,The Fourth Military Medical University, Xi'an 710032, China;Cloning of mutA and expression of its coding protein[J];Journal of Practical Stomatology;2005-03
3 Shi X, Ni LX, Tian Y, Niu ZY, Shi SG, Zhang ZF. 1Military Center for the Diagnosis and Cure of Oral Disease, the 306 Hospital of Chinese PLA, Beijing 100101, China; 2Department of Operative Dentistry and Endodontics, Stomatology College, the Fourth Military Medical University of Chinese PLA, Xi'an 710032, Shaanxi Province, China;Prokaryotic expression of mutacin Ⅰ gene segments from Streptococcus mutan CH43[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-46
4 CHEN Liang, NI Long-xing, ZHANG Hu-ming, CHEN Xi-hua, TONG Zhong-chun(School of Stomatology, Fourth Military Medical University, Xi′an 710032, China);Study of antimicrobial effects of synthetic peptide mutacin from Streptococcus mutans in vitro[J];Chinese Journal of Conservative Dentistry;2007-06
5 LI Song1,LIU Tian-jia2,HU Xiao-yan1,YANG Liu1,LIU Wen1(1.College of Stomatology,Anhui Medical University,Hefei 230032,China;2.West China College of Stomatology,Sichuan University,Chengdu 610041,China);A study on relationship between genotypic diversity and mutacin activity of mutans streptococci[J];Chinese Journal of Microecology;2007-03
6 YANG Liu1,XU Xiao-fang1,HE Hai-hui2,LIU Wen1,LI Song1(1.College of Stomatology,Anhui Medical University,Hefei 230032,China;2.School of Life Science,University of Science and Technology of China,Hefei 230027,China);A comparative evaluation on mutacin of Streptococcus mutans isolates from caries-active against Streptococcus oralis[J];Chinese Journal of Microecology;2008-04
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 LI Li, ZHOU Xue-dong.(State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China);Bacteriocins produced by caries-related bacteria[J];International Journal of Stomatology;2009-01
2 Yang Deqin, Liu Tianjia, Cao Fuxian,et al. Department of Oral Medicine, West C hina College of Stomatology, Sichuan University, Chengdu 610041;An experimental study of gene vaccines pcDNA3-pac and pcDNA3-gtfB of Strept ococcus mutans against dental caries in rats:the influence on weight[J];Journal of Practical Stomatology;2003-06
3 Yang Deqin,Liu Tianjia,Cao Fuxian,et al.Department of Operative Dentistry and En dodontics, West China College of Stomatology, Sichuan University,Chengdu 610041, China;An experimental study on gene vaccines of Streptococcus mutansagainst dental caries in rats:Keyes caries score[J];Journal of Practical Stomatology;2004-05
4 PENG Lei, LIU Xiaodi, GUO Lihong. Department of Oral Biology, School and Hospital of Stomatology, Peking University, Beijing 100081, China;High-level expression and purification of ComE in Escherichia coli[J];Journal of Practical Stomatology;2009-02
5 CHEN Liang, NI Long-xing, ZHANG Hu-ming, CHEN Xi-hua, TONG Zhong-chun(School of Stomatology, Fourth Military Medical University, Xi′an 710032, China);Study of antimicrobial effects of synthetic peptide mutacin from Streptococcus mutans in vitro[J];Chinese Journal of Conservative Dentistry;2007-06
6 YANG Liu1,XU Xiao-fang1,HE Hai-hui2,LIU Wen1,LI Song1(1.College of Stomatology,Anhui Medical University,Hefei 230032,China;2.School of Life Science,University of Science and Technology of China,Hefei 230027,China);A comparative evaluation on mutacin of Streptococcus mutans isolates from caries-active against Streptococcus oralis[J];Chinese Journal of Microecology;2008-04
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LI Song, LIU Tianjia, ZHUANG Heng, et al. (Department of Operative Dentistry and Endodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China);Detection of the Transmitted Strains and Non-transmitted Strains of Mutans Streptococci by AP-PCR[J];West China Journal of Stomatology;2003-05
2 YANG Hai-jie, ZHANG Jun, LUO Wen-xin, LI Shao-wei, GUAN Bao-quan, XIA Ning-shao (The Key Laboratory of Minister of Education for Cell Biology and Tumor Cell Engineering of Xiamen University, Xiamen 361005, China);Construction and application of an efficient, stable secretion prokaryotic expression vector[J];Letters in Biotechnology;2003-06
3 Ding Yun, Ling Junqi, Chen Han. Guanghua College of Stomatology,Sun Yat-sen University, Guangzhou 510055;A study of peptide vaccine HDS from Streptococcus mutans glucosyltransferase——the synthesis and appraisement of peptide HDS[J];Journal of Practical Stomatology;2003-04
4 LI Song1,LIU Tian-jia2,HU Xiao-yan1,YANG Liu1,LIU Wen1(1.College of Stomatology,Anhui Medical University,Hefei 230032,China;2.West China College of Stomatology,Sichuan University,Chengdu 610041,China);A study on relationship between genotypic diversity and mutacin activity of mutans streptococci[J];Chinese Journal of Microecology;2007-03
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