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《Chinese Journal of Microecology》 2012-01
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The whole genome sequence of HBV before and after Lamivudine combined with adefovir dipivoxil therapy in chronic hepatitis B patients

XIANG Hua-guo1,YANG Xiao-li1,PU Rong2,GUO Jiao-xian1(1.Shenzhen Municipal Fuyong People's Hospital,Shenzhen 518103,China;2.Liaobu Hospital of Dongguan City,Dongguan 523400,China)  
Objective To observe the variation of Hepatitis B Virus(HBV) genome before and after treatment of adefovir dipivoxil for rebounded load of HBV after treatment of Lamivudine.Method HBV DNA was extracted from the sera of chronic hepatitis B patients,with one serum sample taken from patients who received Lamivudine for 24 weeks,and the other sample taken from the same patients who then received adefovir dipivoxil for 24 weeks.The whole genome of HBV DNA was sequenced after amplification by polymerase chain reaction.Result By comparing the two whole genome sequences of HBV DNA,we found 29 mutations of amino acids,including 5 in S gene(17.2%),12 in C gene(41.3%),6 in P gene(20.6%),and 6 in X gene(20.6%).In P gene,two amino acids sites'(173 and 204) mutations were related to Lamivudine,but the other four sites'(181,214,236 and 237) mutations were related to adefovir dipivoxil.Conclusion Use of nucleotide analogues is related to drug resistance-associated mutation in HBV genomes.Detection for DNA mutation of HBV genome at regular intervals is significant for reasonable use of nucleotide analogues.
【Fund】: 深圳市科技计划项目(201103042)
【CateGory Index】: R512.62
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