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《Chinese Journal of Microecology》 2012-05
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Screening of high yield gamma-aminobutyric acid producing lactic acid bacteria from 'douchi' and enzymatic properties of its glutamate decarboxylase

GONG Fu-ming1,2,ZHANG Zhong-hua1,SONG Yuan-liang1,LIU Chen-jian1(1.Laboratory of Applied Microbiology,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;2.Basic Department,Dehong Vocational College,Mangshi,678400,China)  
Objective To isolate and screen high yield gamma-aminobutyric acid(GABA) lactic acid bacteria(LAB) from Yunnan traditional fermented soybean food-'douchi' and study the characteristics of its glutamate decarboxylase(GAD) in order to provide references for GABA autofermentation and immobilized production of GAD.Method A high yield GABA-producing lactic acid bacteria was screened by high performance liquid chromatography(HPLC) and its GAD properties were studied.Result A high yield GABA-producing strain,Lactobacillus plantarum YM-4-3,was successfully obtained(the yield of GABA was 5.74 mmol/L or 0.592 g/L).And the optimal fermented conditions for GAD secreting was as follows: 96 hours at initial pH 6.0 and 35 ℃ under anaerobic condition when monosodium glutamate(MSG) concentration was 3%.The enzymatic property study on crude GAD showed that the GAD secreted by L.plantarum YM-4-3 was an acidic enzyme,and it′s activity was optimal at pH 4.0 and 40 ℃,when pyrodoxal-5'-phosphate(PLP) concentration was 200 μmol/L.ConclusionThe traditional fermented 'douchi' from Yunnan province is an potential source for screening LABs with high ability of GABA conversion.This findings will be useful for the RD of new technology for fermented 'douchi' by functional lactic acid bacteria.
【Fund】: 云南省科技厅面上项目(KKSA200926038)
【CateGory Index】: TQ922
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Chinese Journal Full-text Database 5 Hits
1 YANG Sheng-yuan1,2,LU Zhao-xin1,JIAO Yang1,AI Jia1,L Feng-xia1,BIE Xiao-mei1 (1 College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China; 2 College of Life Science and Technology,Guangxi University,Nanning 530005,China);Determination of γ-aminobutyric acid in broth of fermentation by high-performance liquid chromatography[J];Journal of Guangxi Agricultural and Biological Science;2007-04
2 YANG Sheng-yuan1,2,LU Zhao-xin1,*,LU Feng-xia1,BIE Xiao-mei1 (1.College of Food Science and Technology, Nanjing Agriculture University, Nanjing, 210095,China; 2.College of Life Science and Technology, Guangxi University, Nanning, 530005,China);Research Progress on Microbial Glutamate Decarboxylase[J];Food Science;2005-09
3 YANG Sheng-yuan1,2,LU Zhao-xin1,,LU Feng-xia1, BIE Xiao-mei1 (1.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2.College of Life Science and Technology, Guangxi University, Nanning 530005, China);Research Progress Review on Microbial Glutamate Decarboxylase[J];Food Science;2007-01
4 XU Jian-jun,JIANG Bo~*,XU Shi-ying (School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China);Purification and Partial Characterization of Glutamate Decarboxylase from Lactococcus lactis[J];Journal of Wuxi University of Light Industry;2004-03
5 SUN Sen,SONG Jun-mei,QU Jing-ran(College of Food and Biology Engineering,Shandong Institute of Light Industry,Jinan250353);The change of microorganisms in the later fermentation of Dou-chi[J];China Food Additives;2008-02
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ying et al(Key Laboratory of Industrial Microbiology,Ministry of Education,Bioengineering College,Tianjin University of Science and Technology,Tianjin 300457;Department of Biology and Environmenlal Engineering,Tianjin Vocational Institute,Tianjin 300410);Influences of Cell-wall Breaking Methods on Extraction Effect of Glutamate Decarboxylase from Lactobacillus brevis[J];Journal of Anhui Agricultural Sciences;2011-34
2 JIN Hong-xing et al(School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130);Research Progress on the Synthesis of γ-Aminobutyric Acid by Microbe Fermentation[J];Journal of Anhui Agricultural Sciences;2012-11
3 LU Ping,YU Tong-quan,WANG Shu-ying,WANG Jian-li,YANG Liu(New Technological Laboratory in Agriculture Application in Beijing/Department of Biologieal Technology,Beijing Agricultural College,Beijing 102206,China);A Review on Determination of Protein[J];Journal of Beijing Agricultural College;2006-02
4 ZHENG Hong-fa1,HUANG Huai-sheng1,HUANG Ya-hui2,SU Ben-wen1 (1 Tea Research Institute Hunan Academy of Agricultural Sciences,Changsha 410125,China; 2 College of Horticulture,South China Agricultural University,Guangzhou 510642,China);Effect of Soaking Treatment on γ-Aminobutyric Acid in Tea[J];Tea Communication;2010-01
5 OU Jie1,LI Xiao-bei1,HU Jie-yun2,CHEN Ping2,LIN Lu2,YAN Wei-ling2(1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306;2.Shanghai Food Research Institute,Shanghai 200235,China);Isolation and Preliminary Identification of Specific Spoilage Organisms from Traditional Soybean Products White Bean Curd and Bean Curd[J];Soybean Science;2012-01
6 XU Ke, WU Gang, YU Wen-ping, ZHAO Jin, ZHAO Yi-jun (Hubei Provincial Key Laboratory of Urban Aquatic Environmental Ecology,Central China Normal University,Wuhan 430079,China);Optimal Cultivation Conditions and Fermentation Technology of Alga-lysing Bacterium W5[J];Environmental Science & Technology;2009-02
7 YANG Sheng-yuan1,2,LU Zhao-xin1,L Feng-xia1,BIE Xiao-mei1,JIAO Yang1,SUN Li-jun1(1.College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;2.Biology Department,Hanshan Normal University,Chaozhou 521041,China);Mechanism of the Effects of Neutral Salts on Glutamate Decarboxylase Activity[J];Journal of Molecular Catalysis;2007-06
8 SHEN Qiang,LUO Xian-yang,PAN Ke,ZHENG Wen-jia(Guizhou Tea Research Institute,Guiyang,Guizhou 550006,China);Research Progress in Functional Tea Enriched with Gamma Aminobutyric Acid for Decreasing Blood Pressure[J];Guizhou Agricultural Sciences;2011-10
9 ZHANG Xiang-kui,TAN Shu-ming(College of Life Science,Guizhou University,Guiyang,Guizhou 550025,China);Preparation Process Optimization of Polypeptides from Egg White Protein by Enzymatic Hydrolysis[J];Guizhou Agricultural Sciences;2011-10
10 LI Yun, YANG Sheng-yuan, CHEN Yu-na, LIU Xiang-liu, MAI Zhen-zhen (Department of Biology, Hanshan Normal University, Chaozhou 521041, China);Biotransformation for γ-aminobutyric acid production by Enterococcus faecium HS3 cells[J];Guangdong Agricultural Sciences;2010-06
China Proceedings of conference Full-text Database 3 Hits
1 LI Yun, YANG Sheng-yuan* (Food & Fermentation Engineering Institute of the Department of Biology, Hanshan Normal University, Chaozhou 521041);Production ofγ-aminobutyric acid by Pediococcus pentosaceus HS2[A];[C];2009
2 ZHENG Hong-fa, HUANG Ya-hui, HUANG Huai-sheng ( Hunan Tea Research Institute, Changsha 410125, China);Extraction,Separation and Determination of γ-Aminobutyric Acid in Tea[A];[C];2006
3 ZHENG Hong-fa1,HUANG Huai-sheng1,HUANG Ya-hui2,SU Ben-wen1 (1.Huan Tea Research Institute,Changsha,410125,China; 2.College of Horticulture, South China Agricultural University, Guangzhou 510642, China);Effect of Soaking Disposal on γ-Aminobutyric Acid in Tea[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 GUO Xiao-na1, ZHU Yong-yi1, ZHU Ke-xue2 (1.Zhengzhou Institute of Technology, Zhengzhou450052, China; 2.School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China);Study on GABA in Organism[J];Amino Acids & Biotic Resources;2003-02
2 LIU Qing, YAO Hui-yuan, ZHANG Hui (School of Food Science and Technology,Southern Yangtze University,Wuxi 214026,China);Studies on Screening for γ-Amino Butyric Acid Producer and Optimization of Fermentation Conditions[J];Amino Acids & Biotic Resources;2004-01
3 LIN Zhi 1, Masashi Omori 2 (1. Key Lab of Tea Chemical Engineering, Ministry of Agriculture, Tea Research Institute, CAAS, Hangzhou 310008, China; 2. Otsuma Women's University, Chiyoda-ku, Tokyo 102-8357, Japan);Study on the Functional Mechanism of Gabaron Tea on Hypertension[J];Journal of Tea Science;2001-02
4 LIN Zhi1, Masashi Omori2 (1. Key Lab of Tea Chemical Engineering, Ministry of Agriculture, Tea Research Institute, CAAS, Hangzhou 310008, China; 2. Otsuma Women抯 University, Chiyoda-ku, Tokyo 102-8357, Japan);Effects of Gabaron Tea Components on AngiotensionⅠ-Converting Enzyme Activity in Rat[J];Journal of Tea Science;2002-01
5 WEI Xi-hui~1, QI Xing-gui~2, XIA Dong~1, Jiang Jing-hu~1 (1.College of Animal Science and Technology, Nanjing Agric Univ, Nanjing 210095, China;2.JiangSu Huian Station of Animal Husbandry and Vet, Huaian 224100, China);Effect of Dietary γ-aminobutyric Acid on Finishing Hogs Growth and Forage Application[J];Ecology of Domestic Animal;2004-02
6 LIU Jian Fang,HOU Yan Ning,LIU Hui Chen (Department of Clinical Pharmacology,Bethune International Peace Hospital of PLA,Shijiazhuang 050082,Hebei);Determination of Glutamate and γ-Aminobutyric Acid in Rat Brain by HPLC with Pre Column Derivation and Electrochemical Detection[J];Pharmaceutical Journal of Chinese People's Liberation Army;2000-06
7 Guo Xiaona,Zhu Yongyi;Application of Response Surface Methodology (RSM) in the Study of Germinated Brown Rice[J];Cereal & Feed Industry;2003-11
8 Shu Ling BIAN 1 , Wei ZHANG 1 , Hui ZHU 1 , Jiang NI 1 , Lan Chun YAO 1 , Liang CHEN 2 (1.Department of Physiology, Harbin Medical University, Harbin, Heilongjiang 150086, China; 2.Department of Inferitile, the Second Affilia;Effect of γ-Aminobutyric Acid on the Sperm Acrosin Activity[J];Acto andrologica Sinica;2002-05
9 Bian Shuling,Zhang Wei,Zhu Hun,et al Department of Physiology, Harbin Medical University,(Harbin 150086);Effect of GABA on the sperm acrosome reaction in antisperm antibody positive patients[J];Chinese Journal of Andrology;2002-05
10 Zhao Jinglian(Xian Jiaotong University,Xian);STUDIES ON PRODUCTION OF γ-AMINOBUTYRIC ACID BY IMMOBILIZED ESCHERICHIA COLI CELLS[J];Chinese Journal of Biotechnology;1989-02
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