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《Chinese Journal of Microecology》 2012-05
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Mechanism of killing action by Herba schizonepetae on Trichomonas vaginalis in vitro

DAI Xiao-dong1,QIN Yuan-hua1,LIU Xin2,ZHENG Li-li1,REN Yi-xin1,CUI Yu1(1.Department of Parasitology,Dalian Medical University,Dalian 116044,China;2.Department of Microbiology,Dalian Medical University,Dalian 116044,China)  
Objective To explore the mechanism of killing action by Herba schizonepetae on Trichomonas vaginalis in vitro.Method 1:4 concentration of Herba schizonepetae water extract(HSWE) was added to the culture media of T.vaginalis in vitro.After treated with HSWE for 2 h and 4 h respectively,the ultrastructural changes of T.vaginalis were observed by transmission electronic microscope.Result After treated with HSWE,T.vaginalis exhibited depolymerization of polyribosomes,degranulation of rough surfaced endoplasmic reticula and increment of vacuoles in cytoplasm.With the prolongation of drug action time,incomplete nuclear membranes and raritas karyoplasm appeared.Eventually nuclear membranes ruptured,their cellular content overflew,and then parasites died.Conclusion Herba schizonepetae can destroy the endomembrane system of Trichomonas and thus exhibits a relatively potent killing effect on T.vaginalis.
【CateGory Index】: R285.5
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【Citations】
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1 YAN Yan, GAO Xing-zheng (Department of Parasitology, School of Basic Medical Science, Peking University, Beijing 100083, China);TRICHOMONACIDAL EFFECT AND SCANNING ELECTRON MICROSCOPE STUDY OF PULSATILLA CHINENSIS AGAINST TRICHOMONAS VAGINALIS IN VITRO[J];Chinese Journal of Parasitic Disease Control;2003-06
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