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《Chinese Journal of Microecology》 2012-09
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Study on protective reagent and techniques for active microbial agents of Lactobacillus acidophilus

DAI Hai-bing1,WANG Han1,DAI Hai-ping2,XU Bo2(1.Mudanjiang Medical College,Mudanjiang 157011,China;2.Hongqi Hospital Affiliated to Mudanjiang Medical College,Mudanjiang 157011,China)  
In order to improve the survival rate of Lactobacillus acidophilus and prolong its validity,we optimized the protective agents in different technological processes.First we screened out the important factors affecting the live bacteria survival rate by Plackett-Burman design during the cultivation of the Lactobacillus acidophilus and tablet compression.Then we determined the optimal level of the factors using uniform design.Confirmatory experiments proved that addition of sodium chloride 0.3%,mannitol 0.4%,maltose 3.5%,ascorbic acid 1.0% and sucrose 35.0% increased the live bacteria survival rate virtually.
【CateGory Index】: TQ463
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