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《Chinese Journal of Microecology》 2014-01
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Analysis of the alarm time by automated blood culture system with clinical specimens

DA Rong;WANG Wei;MA Chen;JU Xi-chi;Department of Clinical Laboratory, the First Affiliated Hospital of Xi'an Jiaotong University;Department of Neurosurgery, the First Affiliated Hospital of Xi'an Jiaotong University;Department of Neurology, the First Affiliated Hospital of Xi'an Jiaotong University;  
Objective To investigate the alarm time by automated blood culture system with different types of pathogens. Methods Blood bottles from September 9th to November 10th 2013 in our hospital were collected; the specimens were detected by BacT / ALERT 3D automated blood culture system. The data of alarm time of the bottles were analyzed using SPSS 18. 0. Results Of the total 4,050 bottles collected,449( 12. 47%) were positive. The average alarm times of aerobic,anaerobic and pediatric bottles were( 0. 80 ± 0. 05) day,( 0. 70 ± 0. 05) day and( 0. 99 ±0. 10) day,respectively. The alarm time of anaerobic bottles was significantly different from that of pediatric bottles( P 0. 05). For aerobic bottles,the alarm time was 1. 03 ± 0. 08 days for gram-positive bacteria, 0. 62 ± 0. 05 day for gram-negative bacteria,1. 35 ± 0. 36 days for fungi,and 0. 48 ± 0. 08 day for mixed infection, while for anaerobic bottles,it was 0. 97 ± 0. 11 day( gram-positive bacteria),0. 62 ± 0. 07 day( gram-negative bacteria),and 0. 44 ± 0. 09 day( mixed infection) respectively. The alarm time of pediatric bottles for gram-positive bacteria,gram-negative bacteria,fungi,and mixed infection were 1. 09 ± 0. 12 days,0. 47 ± 0. 08 day,1. 79 ± 0. 60 days,and 1. 45 days respectively. Significant differences were seen between the alarm time for gram positive bacteria and those for gram negatives and mixed infection( P 0. 05). Conclusion The alarm time for grampositive bacteria is longer than those for gram-negative bacteria and mixed infection. Double bottle with double unit can improve the positive rate of blood culture,and provide evidence for targeted antibiotics use.
【Fund】: 国家自然科学基金资助项目(81000767)
【CateGory Index】: R446.5
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