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《Chinese Journal of Microecology》 2015-01
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Screening and identification of Lactic acid bacteria in 30 week old caged Hyline brown laying hens

WANG Qiu-ju;CUI Yi-zhe;LIU Hong-chen;GAO Yan;GUO Zhao-zhou;ZHANG Shi-bo;HE Yang;LIU Zhao-peng;College of Animal science and Veterinary,Heilongjiang Bayi Agricultural University;  
Objective This experiment mainly carries on screening and identification of lactic acid bacteria in the caecum of layers. Methods 10 lactic acid bacteria isolated from cecum contents of laying hens fed in cages were determined on ability of acid production,acid resistance and bile salt resistance,to obtain the high quality LAB to determine their growth curves,acid production and ability of anti-drugs,and API CHL50 were conducted to identify the bacteria family. Results All p H of 10 strains decreased significantly from the initial p H 6. 1- 6. 4 after fermentation for 48 h. With the culture p H decreased or the culture bile salt concentration increased and treated time delayed,the survival rate of every strain decreased. CL1,CL12 and CL54 grown faster and the best fermentation time was 24 h,while CL26 and CL31 grown slower a little and the best fermentation time was 32 h. CL1,CL12 and CL54 had higher ability to anti-drugs,while CL26 and CL31 had lower activity to anti-drugs. Conclusion CL1,CL12 and CL54 were selected and identified by API CHL50,results show that CL1 is Lactococcus Lactis ssp Lactis1,CL12 is Pediococcus Acidilactici,and CL54 is Lactobacillus Brevis1.
【Fund】: 黑龙江省教育厅大学生创新项目(201310223014);; 黑龙江省教育厅面上项目(12531463);; 黑龙江省应用技术研究与开发计划项目(WB13B11101)
【CateGory Index】: S831
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Chinese Journal Full-text Database 9 Hits
1 WANG Qiu-ju,CUI Yi-zhe,ZHU Shuang,LIU Sheng-jun,ZHOU Rui-jin(College of Animal Science and Veterinary,Heilongjiang Bayi Agricultural University,Daqing 163319,China);Isolation and identification of Lactic acid bacteria in jejunum of laying hens fed in cage[J];中国微生态学杂志;2013-05
2 XIANG Xin1,LI Xiao-ran1,CHEN Hong2,LUAN Jian-jun1,LUO Yi-yong1,LIU Chen-jian1(1.Laboratory of Applied Microbiology,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;2.Department of Gynecology,First People's Hospital of Yunnan Province,Kunming 650034,China);Isolation and screening of lactic acid bacteria with antimicrobial activity from female reproductive tracts and analysis of antimicrobial substances[J];中国微生态学杂志;2013-03
3 GU Tian-tian et al(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong 266109);Separation and Identification of Lactobacillus from the Intestinal Tract of Healthy Piglet[J];安徽农业科学;2013-02
4 HU Shunzhen ZHANG Jianmei* XIE Quanxi LI Xiaoying(Shandong Boly-Lely Bioengineering Co.,Ltd.,Tai'an 271000,China);Effects of Compound Probiotics on Performance,Intestinal Microflora, Antioxidant Indices and Immune Function of Broilers[J];动物营养学报;2012-02
5 ZHOU Xiao-ying,CHEN Xiao-lin(College of Life Science,Anhui Agriculture University,Hefei 230036,China);The beneficial function of Lactic acid bacteria and research progress in its application[J];中国微生态学杂志;2011-10
6 REN Da-yong1,2,LI Chang2,QIN Yan-qing1,2,JIN Ning-yi2(1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China;2.Military Veterinary Institute,Academy of Military Medical Sciences,Changchun 130118,China);Development Review on Healthy Function and Potential Mechanisms of Lactic Acid Bacteria[J];中国兽药杂志;2011-02
7 YANG Xia,CHEN Lu,WANG Xin-wei,ZHANG Hong-qu,CHANG Hong-tao,LIU Hong-ying,WANG Chuan-qing(Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China);Study on isolation and biological characteristics of Lactobacillus in intestines of pig[J];中国微生态学杂志;2010-05
8 LIU Hongjie~1,CHENG Liangui~2,NIU Zhongxiang~1,WANG Jianchang~1,NING Haiqiang~1 ~1College of Animal Science and Technology,Shangdong Agricultural University,Tai′an 271018, China;~2Department of Bioenginecring,Binzhou Vocational College,Binzhou 256624,China);Comparison of the quantity of normal flora in digestive tracks betweeol SPF and ordinary chicken[J];生态学杂志;2005-07
9 ZHAO Rui-xiang~1,SUN Jun-liang~1,LI Yuan-rui~2,YUAN Zhi-fa~2 (1 Food College,Henan Vocation-Technical Teachers College,Xinxiang,Henan 453003,China; 2 College of Food Science and Engineering,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Study on the intolerance ability of Lactobacillus acidophilus on pH and bile salt[J];西北农林科技大学学报(自然科学版);2004-02
Chinese Journal Full-text Database 10 Hits
1 Tang Yizhong;;The effect analysis of lactic acid bacteria in the vagina capsule in treating cervical erosion combined the curative effect of chronic cervicitis[J];浙江临床医学;2016-04
2 Xu Chao;Gao Han;Su Xiaoming;Liu Wei;Liu Weizhen;Yu Liyun;College of Life Science and Technology,Heilongjiang Bayi Agricultural University;;Study on Fermentation Process of Recombinant Lactobacillus casei[J];黑龙江八一农垦大学学报;2015-06
3 CAO Zhen-hui;LIU Yong-shi;PAN Hong-bin;TIAN Rui-jiao;GU Da-hai;LI Shu-ying;XU Yong-ping;LIN Qiu-ye;Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science;Faculty of Animal Science and Technology,Yunnan Agricultural University;College of Food Science and Technology,Yunnan Agricultural University;Post-doctoral Research Workstation Dalian SEM Bio-Engineering Technology Co.,Ltd.;School of Life Science and Technology,Dalian University of Technology;;Research progress of Lactic acid bacteria on the probiotic properties and mechanisms[J];食品工业科技;2015-24
4 WANG Hehua;PAN Boyu;HUANG Juan;CHEN Xiaoping;WU Yuanxin;Key Laboratory for Green Chemical Process of Ministry of Education/School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology;;Activity and Stability Study of Aldehyde Dehydrogenase 2(ALDH2)Expressed by Prokaryotes[J];氨基酸和生物资源;2015-04
5 CHAI Jian-hua;CHANG Hong-mei;SHU Hong-wen;LI Lian;Chongzhou People's Hospital;;Lacidophilin Vaginal Capsules reduce the recurrence of vulvovaginal candidiasis:a Meta analysis of Chinese literatures[J];中国微生态学杂志;2015-10
6 WANG Xiaowen;JIANG Caihong;LU Shiling;LU Wenjuan;DU Zixuan;LI Xu;LI Baokun;Food College, Shihezi University;;Research on mutagenesis of ethanol tolerance lactic acid bacteria[J];中国酿造;2015-10
7 CHENG Long;GONG Fu-ming;LI Xiao-ran;XIANG Xin;LUO Yi-yong;LIU Chen-jian;Faculty of Life Science and Technology, Kunming University of Science and Technology;Basic Medical Department, Dehong Vocation College;;Screening and Potential Probiotic Properties of Lactic Acid Bacteria with High Angiotensin-I Converting Enzyme-inhibition Activity[J];现代食品科技;2015-11
8 Shi Ying;Hu Yin-rong;Li Jing;Guo Ying;Li Yang;Xu ri-hua;College of Life Science, Inner Mongolia University;;Detection of EPS producing bifidobacterium by polymerase chain reaction[J];生物技术世界;2015-08
9 Yajing CHAI;Hao SHI;Ri NA;College of Physical Science and Technology, Inner Mongolia University;;Identification and Mutagenesis of Lactic Acid Bacteria from Chinese Sauerkraut[J];Agricultural Science & Technology;2015-07
10 LIU Si-Tong;WANG Hong-Ning;MA Bing-Cun;DING Meng-Die;CAO Hai-Peng;YANG Xin;Animal Disease Prevention and Food Safety Key Lab of Sichuan Province,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University;;Isolation and identification of lacticacid bacteria from Tibetan chicken and expression of foreign protein in it[J];四川大学学报(自然科学版);2015-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HUANG Wen-qiang et al(Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Research,Taizhou,Jiangsu 225300);Study on Isolation and Biological Characteristics of Lactobacillus in Intestines of Langshan Chicken[J];安徽农业科学;2012-25
2 WU Xiang-yu, XU Hai-yan, XIN Guo-qin, CAO Yin-sheng, GU Wei (Shandong Baolai-leelai Bioengineering Co. Ltd., Tai′an 271000, China);Isolation,Identification and Biological Characteristics of Lactic Acid Bacteria[J];畜牧与饲料科学;2012-03
3 LIU Xuemei,LUAN Shuli,WEN Jianxin(College of Animal Science and Technology,QAU,Qingdao 266109,China);The Separation of Lactic Acid Bacteria in Healthy Domestic Rabbit Intestinal[J];青岛农业大学学报(自然科学版);2011-01
4 Yuan Na,Chen Qi,Liu Congmin,Zhao Baokai,Zhang Zhigang;Effect of the compound microbial eclogical agents on NH_3's concentration,nutrient digestibibity and intestinal flora of breeding layers[J];饲料工业;2010-20
5 Xiaodong Chu1,Yuheng Lin2,Zhizeng Sun2,Liandong Huan2,Jin Zhong2(1College of Life Sciences,Capital Normal University,Beijing 100048,China)(2lnstitute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China);Advances in the study of nisin Resistance——A review[J];微生物学报;2010-09
6 CHEN Jiaxiang ZHANG Renyi WANG Quanxi FU Zhicai ZHENG Qiaoxia WANG Changkang(College of Animal Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China);Effects of Bacillus licheniformis on Growth Performance,Antioxidant Indices and Blood Biochemical Parameters of Broiler Chickens[J];动物营养学报;2010-04
7 ZHANG Shiyuan1 XU Chunyan1 DONG Xiaofang2 TONG Jianming2 ZHANG Qi2 (1. Institute of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China; 2. State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Effects of Water Soluble Alfalfa and Astragalus Polysaccharide on Proliferation of Lymphocyte in Broilers[J];动物营养学报;2010-03
8 CAI Dong-lian1, WU Pei-ying2, YANG Zhen-nan3, DING Li-wei3, YE Xue-fei3. 1.Department of Nutrition, Affiliated Changhai Hospital of Second Military Medical University, Shanghai 200433, China; 2.Department of Nutrition, Shanghai First People’s Hospital, Shanghai 200080, China; 3.R&D, Hangzhou Wei-Chuan Foods Co, Ltd, Hangzhou 310018, China;Study on evaluating the promote effect of Wei-Chuan Active Lactobacillus Drinks on physical intestinal canal health[J];中华疾病控制杂志;2010-06
9 LIU Jing1,2,HUANG Shan-shan1,ZHAO Zheng1(1.College of Food Engineering and Biotechnology,Tianjin University of Science and Technology Tianjin 300457,China;2.College of Bioscience and bioengineering,Hebei University of Economics and Business Shi-Jiazhuang 050061,China);Research on antioxidative capacity of lactic acid bacteria[J];中国乳品工业;2010-05
10 YAO Wei-qi,CHEN Mao-bin,ZHEN Da,GUO Yi-shan;Isolation of Lactate-producing Microbes From Fermented Grains of Luzhou-flavor Liquor and Their Effect on Simulative Solid-state Fermentation[J];酿酒;2010-03
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