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《Chinese Journal of Microecology》 2015-01
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Screening and identification of Lactic acid bacteria in 30 week old caged Hyline brown laying hens

WANG Qiu-ju;CUI Yi-zhe;LIU Hong-chen;GAO Yan;GUO Zhao-zhou;ZHANG Shi-bo;HE Yang;LIU Zhao-peng;College of Animal science and Veterinary,Heilongjiang Bayi Agricultural University;  
Objective This experiment mainly carries on screening and identification of lactic acid bacteria in the caecum of layers. Methods 10 lactic acid bacteria isolated from cecum contents of laying hens fed in cages were determined on ability of acid production,acid resistance and bile salt resistance,to obtain the high quality LAB to determine their growth curves,acid production and ability of anti-drugs,and API CHL50 were conducted to identify the bacteria family. Results All p H of 10 strains decreased significantly from the initial p H 6. 1- 6. 4 after fermentation for 48 h. With the culture p H decreased or the culture bile salt concentration increased and treated time delayed,the survival rate of every strain decreased. CL1,CL12 and CL54 grown faster and the best fermentation time was 24 h,while CL26 and CL31 grown slower a little and the best fermentation time was 32 h. CL1,CL12 and CL54 had higher ability to anti-drugs,while CL26 and CL31 had lower activity to anti-drugs. Conclusion CL1,CL12 and CL54 were selected and identified by API CHL50,results show that CL1 is Lactococcus Lactis ssp Lactis1,CL12 is Pediococcus Acidilactici,and CL54 is Lactobacillus Brevis1.
【Fund】: 黑龙江省教育厅大学生创新项目(201310223014);; 黑龙江省教育厅面上项目(12531463);; 黑龙江省应用技术研究与开发计划项目(WB13B11101)
【CateGory Index】: S831
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