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《Chinese Journal of Microecology》 2015-01
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The inhibitory effect of grape seed proanthocyanidins on Candida albicans in vitro

DONG Bo;DIAO Tian-jiao;LI Yi-ling;LI Dong-mei;FU Yue-ming;The Affiliated Stomatological Hospital of Jiamusi University;Jiamusi University School of Stomatology;  
Objective To study the inhibitory effect of grape seed proanthocyanidins on Candida albicans by observing its impact on biofilm formation,and explore its role in the changes of oral microflora. Methods MTT assay was used to determine the minimum inhibitory concentrations of grape seed proanthocyanidins against Candida albicans in vitro. Confocal laser scanning microscope( CLSM) was used to observe the effects of different concentrations of the drug. Red and green fluorescence staining was performed for quantitative analysis. Results The minimum inhibitory concentration was 32 mg / m L. The inhibitory effect increased along with the concentration of the drug within the scope of the concentration being tested. CLSM showed that grape seed proanthocyanidins reduced the proportion of viable cells in Candida albicans biofilm and inhibited the activity of the biofilm. Conclusion Grape seed proanthocyanidins can significantly inhibit Candida albican in vitro.
【CateGory Index】: R781.3
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