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《Chinese Journal of Microecology》 2015-10
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Paeruginosa exotoxin A induced oxidative stress triggers autophagy in mouse lung epithelial 12 cells

WANG Ting-ting;KANG Si-jing;HU Tian-yu;Department of Geriatrics,the First Affliliated Hospital of China Medical University;Department of Hematology,the First AffliliatedHospital of China Medical University;Second Department of Medical Oncology,Shengjing Hospital Affiliated of China Medical University;  
Objective To investigate the influences of Paeruginosa exotoxin A(PEA)on oxidative stress injury and autophagy activity of MLE-12 cells.Methods MLE-12 cells in logarithmic phase were treated with PEA at different concentrations for 1or 2hours.Furthermore,the MLE-12 cells were exposed to PEA at the concentration of 1,000ng/mL for different length of time.The production of H2O2 was quantified with Hydrogen Peroxide Assay Kit.The expression of LC3 protein was examined by Western blot and LC3Ⅱ/β-actin ratio was measured,respectively.Results Compared with the control group,the PEA treated cells showed significant increases of H2O2 with the increased concentration of PEA.The production of H2O2 were increased by 1.73 times,2.09 times,3.83 times and 5.06 times respectively compared with the control group after stimulated by PEA for 2hours(P0.01).At the same time,the expression of autophagy marker LC3Ⅱ was also significantly increased.Moreover,there was a significant rise in both H2O2 and LC3 II after the cells were treated with high concentration of PEA(1,000ng/mL)along with the time extended.Conclusion PEA can induce oxidative stress injuries and activate cell autophagy activity in MLE-12 cells.
【CateGory Index】: R363
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【Citations】
Chinese Journal Full-text Database 3 Hits
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 YOU Jia-feng;ZHENG Wei-chao;DENG Yun;Medical School,Anhui University of Science and Technology;;Macrophage autophagy mediated by intracellular expression of Dectin-1 in Aspergillus fumigatus infection[J];中国微生态学杂志;2016-03
2 WANG Ting-ting;KANG Si-jing;HU Tian-yu;Department of Geriatrics,the First Affliliated Hospital of China Medical University;Department of Hematology,the First AffliliatedHospital of China Medical University;Second Department of Medical Oncology,Shengjing Hospital Affiliated of China Medical University;;Paeruginosa exotoxin A induced oxidative stress triggers autophagy in mouse lung epithelial 12 cells[J];中国微生态学杂志;2015-10
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1 YANG Xiao-kang;DAI Jing-jing;QI Zhen-yu;HAO Shuai;WANG Gan-xun;WANG Li-yu;HU Dong;ZHANG Rong-bo;Department of Immunology and Laboratory Medicine,Medical School,Anhui University of Science and Technology;Pathology Deparment of the General Hospital of Oriental Group;;The impact of different shapes of Aspergillus fumigatus on the level of macrophage autophagy[J];中国微生态学杂志;2014-05
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