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《Chinese Journal of Microecology》 2015-10
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Comparison of real-time PCR and conventional bacterial identification method in detection of intestinal pathogenic bacteria

TIAN Mei;SHEN Jing;Department of Laboratory Medicine,Jiangsu Shengze Hospital;  
Objective To compare and analyze the detection results of Salmonella spp and Shigella spp by qPCR and conventional bacterial identification method.Methods The fecal specimens from 4,662 cases of acute diarrhea patients during 2010 and 2014in Jiangsu Shengze Hospital were detected by using both qPCR and conventional bacterial identification.And all the samples with positive results by qPCR were verified with conventional bacterial identification method.The results were statistically analyzed and summarized.Results 85strains(1.82%)of Salmonella sppand 192strains(4.12%)of Shigella spp were detected by qPCR method,while 81strains(1.74%)of Salmonella sppand 184strains(3.95%)of Shigella spp were detected by conventional bacterial identification method;there was no significant difference between the results of the two methods(P0.05).All the specimens with positive qPCR results were positive by the conventional bacterial identification method.Conclusion The qPCR method is accurate for detection of Salmonella and Shigella with reliable results,and can meet the requirement of clinical diagnosis of intestinal diseases.
【CateGory Index】: R440
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