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《Chinese Journal of Microecology》 2016-01
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Effect of soft excrement and intestinal fluid on the colonization of gut flora in Cold Region Rex Rabbits

REN Chuan-shuai;SHI Qi-jie;ZHANG Zhen;WANG Hao;WANG Hong-zhan;BAI Xiu-juan;College of Animal Science and Technology,Northeast Agricultural University;  
Objective To analyze the variation of Bacteroides,Clostridium,Veillonella and Escherichia coli in the gut flora of Cold Region Rex Rabbits accepting orally soft excrement and intestinal fluid,and explore the effect of soft excrement and intestinal fluid on the colonization of gut flora.Methods Ninety rabbits were divided into Group I,II or III,accepting 1mL of soft excrement,intestinal fluid and purified water,respectively.Then the DNA of bacteria was extracted from the aseptically scraped intestinal contents of duodenum,jejunum,ileum and cecum of 32-and 46-day rabbits.Species specific primers were designed according to the variable region sequences of the 16 SrDNA V3of Bacteroides,Clostridium,Veillonellaand Escherichia coli,and RT-PCR was used to analyze the amounts of different bacteria.Results The concentration of Bacteroides in jejunum as well as those of Clostridium and Veillonella in ileum in group I were higher(5.77E-93.58E-10,1.10E-93.39E-10)(t=8.20,t=2.16,P0.05)and significantly higher(5.27E-63.58E-7,7.41E-61.43E-6,1.57E-51.33E-6,7.99E-61.80E-6)(t=3.07,t=4.24,t=5.87,t=6.28,P0.01)than the control group(32-and 46-days rabbits).The amounts of Clostridium in duodenum and ileum in group II were significantly higher(3.71E-74.87E-8,6.88E-63.58E-7)(t=3.42,t=2.95,P0.01)than in the control group(32-days rabbits),and the amount of Veillonellain ileum in group II was higher(4.13E-61.80E-6)(t=2.03,P0.05)than in the control group(46-days rabbits).Conclusion Soft excrement and Intestinal fluid can promote the colonization of Bacteroides,Clostridiumand Veillonella,and have less effect on Escherichia coli.
【CateGory Index】: S829.1
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