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《Chinese Journal of Microecology》 2017-08
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Screening of candidate probiotic strains based on acid resistance and antibacterial effect

PAN Haoyu;NING Kaiting;LI Peipei;ZHANG Jin;SONG Jiaping;TANG Li;LIU Yinhui;YUAN Jieli;WEN Shu;Dalian Medical University;  
Objective To provide a good candidate strain for the development of probiotics based on the screening of strains with strong acid resistance and antibacterial effect,explore the correlation between acid resistance and antibacterial effect,and develop new method for quick screening of probiotics.Methods 62 different strains of Lactobacilli,Weissella and Enterococcus faecalis were cultivated in the media with different acidity to observe the acid-resistant ability.Escherichia coli and Staphylococcus aureus were used as indicator bacteria,and the antibacterial effect of the tested strains was studied by using Oxford cup method.The correlation between acid resistance and antibacterial ability was analyzed.Results Under the acidic environment,there were 22 strains with a survival rate of over 50%;the strains with strong acid resistance included DMLA16017,DMLA16116,DMLA16117,DMLA16118 and DMLA16131.There were several strains showing a strong inhibitory effect of pathogens,including the strains DMLA16017,DMLA16009,DMLA16118,DMLA16002 and DMLA15015.Conclusion The strain DMLA16017(Lactobacillus rhamnosus)and DMLA16118(Lactobacillus fermentum)showed excellent acid resistance and bacteriostasis.The ability of the strains to inhibit pathogensis correlated with their acid tolerance,which can be used to achieve quick screening of candidate probiotic strains.
【Fund】: 国家自然科学基金(81370113);; 国家“863”计划(2014AA022209);; 国家“973”计划(2013CB531405)
【CateGory Index】: R371
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