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《Chinese Journal of Microecology》 2017-08
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Screening and identification of digestive enzyme-producing bacteria from the intestine of Anomala corpulenta larvae

XUE Zhijing;ZHUANG Guifen;HUNAG Zhendong;ZHANG Ruiling;ZHANG Zhong;Department of Pathogenic Biology of Taishan Medical University;  
Objective To isolate and identify enzyme-producing bacteria from the intestine of Anomala corpulentalarvae and analyze the impact of ecological environment and food on the diversity of the bacteria.Methods Anomala corpulentalarvae were identified from the grubs collected from the wild(wild grubs)and peanut field(peanut-field grubs).Then the intestinal symbiotic bacteria were isolated from the intestine of the grubs with conventional methods.The ability of the bacteria to produce amylase,protease,lipase and cellulase were determined with iodine staining or inhibition zone method.Results 24 strains of bacteria were isolated,including 9 strains from the wild grubs and 15 strains from the peanut-field grubs.Among the enzyme-producing bacteria from the wild grubs,one strain could produce amylase,one could produce cellulase,one could produce lipase,and 4 strains were able to produce protease.Among those from peanut-field grubs,one strain could produce amylase,3 strains could produce protease,9 could produce cellulase;no lipase producing strains were isolated.Conclusion Protease-producing bacteria is predominant in the intestine of the wild grubs,accounting for 44.4%,while cellulase-producing bacteria is predominant in the intestine of peanut-field grubs,which accounted for 60.0%.The composition of digestive enzyme-producing bacteria in the intestine of Anomala corpulenta larvae from different soils reflected the impact of environment and food on the adaptation of the grubs.
【Fund】: 国家自然科学基金(81271874);; 山东省科技发展计划(2014GSF121007)
【CateGory Index】: Q965
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