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《Chinese Journal of Microecology》 2017-08
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Identification of a strain of amylase-producing Bacillus sp. SY200 and its biological antagonism against animal pathogenic bacteria

PAN Wenjie;FENG Jie;YANG Fan;Chengdu No.7 High School Jiaxiang Foreign Language School;  
Objective To identify an amylase-producing strain of Bacillus sp.SY200 and analyze its biological antagonism against animal pathogenic bacteria.Methods The genomic DNA was extracted from Bacillus sp.SY200,then the target 16S rRNA fragments were amplified with the universal primers by PCR.The sequence and alignment analysis were performed using the NCBI with BLAST data base.The system of evolutionary tree was built and analyzed subsequently.Biological antagonism of the basic culture liquid and culture supernatant of the bacteria SY200 against three stains of animal pathogenic bacteria were measured by using filter-paper method and Oxford cup method respectively.Results In combination with the morphological and physiochemical characteristics,the strain SY200 was identified as Bacillus methylotrophicus.The basic culture liquid and culture supernatant of SY200 showed strong biological antagonistic effect against enterotoxigenic Escherichia coli,Salmonella pullorum and Staphylococcus aureus;the antibacterial substances mainly existed in bacterial metabolites.Conclusion Bacillus sp.SY200 is identified as Bacillus methylotrophicus with strong biological antagonism against three strains of animal pathogenic bacteria.
【Fund】: 成都嘉祥外国语学校中学生生物科研兴趣课题;; 四川省高等学校科技创新团队资助项目(动物源性食品安全创新团队KM406183.1)
【CateGory Index】: S852.6
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