Significance of combined detection of hepatitis B virus large surface protein and PreS1 antigen in the diagnosis of HBeAg-negative hepatitis B
ZHANG Suzhen;ZHANG Jie;YU Suan;FU Fengyun;Clinical laboratory,Hangzhou Hospital for the Prevention and Treatment of Occupational Disease;
Objective To explore the significance of combined detection of hepatitis B virus large surface protein(HBV-LP)and PreS1antigen(PreS1-Ag)in the diagnosis of HBeAg-hepatitis B(HB).Methods HBVLP and PreS1-Ag were detected with enzyme linked immunosorbent assay(ELISA),and the expression of HBV-DNA was detected by using quantitative real-time PCR(qRT-PCR).The positive rates of HBV-LP,PreS1-Ag and HBV-DNA were compared among the HB patients with different HBV-marker(HBV-M)models.The positive and negative predictive values of HBV-LP and PreS1-Ag in independent/joint detection for HBeAg-HB patients were analyzed based on the expression of HBV-DNA,which was used as the gold standard for HBV infection and replication.Results(1)In the 115 HBeAg+ cases,the positive rates of HBV-LP and PreS1-Ag were not significantly different from that of HBV-DNA(Ps0.05).In the 120HBeAg-/HBeAb+ HB patients,the positive rate of HBV-LP(64.2%)was obviously higher than that of HBV-DNA(P0.05),but there was no significant difference between the positive rates of PreS1-Ag and HBV-DNA(P0.05).In the 65 HBeAg-/HBeAb-HB patients,the positive rates of HBV-LP(72.3%)and PreS1-Ag(67.7%)were both significantly higher than that of HBV-DNA(Ps0.05).(2)Based on the HBV-DNA expression of 185 HBeAg-HB patients,the positive/negative predictive values of HBVLP and PreS1-Ag were 72.6%/71.6% and 93.4%/84.1%,respectively.The positive rate of HBV-DNA in patients with positive HBV-LP/PreS1-Ag(66.0%)was significantly higher than that in patients with negative HBV-LP/PreS1-Ag(P0.05).Conclusion Serum levels of HBV-LP and PreS1-Ag were related to the expression level of HBV-DNA,combined detection of HBV-LP and PreS1-Ag could sensitively reflect the replication of HBV in HBeAg-HB patients,and predict the level of HBV-DNA.
【CateGory Index】： R446.6;R512.62