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《CHINESE JOURNAL OF MICROECOLOGY》 1998-01
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AN EXPERIMENT STUDY ON THE PLASMIDS FOR STREPTOCOCCUS SANGUIS

Xu qi, Zhang Jinchai,Zhang YunhuiCollege of Stomatology, West China University of Medical Sciences, Chengdu 610041  
Streptococcus sanguis is a `beneficial' species for periodontal health.Plasmids are extrachromosomal genetic elements,which code some proteins with specific functions.The purpose of this study is to detect and characterize any plasmids which may code for antibiotic resistance in S. sanguis,and to provide basis for studying S. sanguis on molecular level. Seven strains of S. sanguis were examined for plasmid presence by a modified alkaline lysis procedure.Plasmid DNA was separated by agarose gel electrophoresis,using λ DNA/Hind Ⅲ as molecular weight marker.The disc method was used for the drug seneitivity test of the 7 straina. Antibiotics tested were tetracycline, streptomycins.chloromycetin and erythromycin.The result showed that 23kb-plasmids were found in 4 of 7 S. sanguis strains. The plasmids were not found in direct association with antibiotic resistence.
【Fund】: 国家教委博士点基金
【CateGory Index】: R378.12,
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