Determination of melamine in milk products by solid phase extraction-high performance liquid chromatography
DING Yang;DENG Mei-rong;NIE Jing;SUN Zhong-hua;Physical and Chemistry Laboratory,Harbin Center for Disease Control and Prevention;
Objective To establish a new method for the detection of melamine of dairy products by solid phase extraction-high performance liquid chromatographic. Methods In this experiment,pretreatment method was improved by many times,the sample was enriched through sep-pak column before entering the cation exchange solid-phase extraction column and was determined by HPLC. Results Through the test of the 100 copies of melamine in dairy and dairy product samples,the minimum detection concentration was 2 mg / kg; the recovery was 90. 5%-94. 3%; relative standard deviation of six determinations( RSD) was 2. 43%. Conclusion Compared with the original national standard method,the improved pretreatment method makes the test time shorter,the result is more accurate,reliable and has a good repeatability,it is realized on the fast and accurate qualitative and quantitative analysis of melamine in dairy and dairy products.
【CateGory Index】： R155.5;O657.72