Liver tissue SF,Hepc,Tf gene expression in obesity rats
ZHU Chun-yang;FENG Li;ZHANG Gui-ying;LIU Ya-juan;Jilin Province Center for Food and Drug Evaluation;
Objective To study the mechanism of iron malnutrition in obesity rats,providing the theoretical foundation for the prevention and therapy of iron malnutrition induced by obesity. Methods 27 male Wistar rats were divided into two groups,the control group( n = 10) and the obesity group( n = 17). The rats were fed with ordinary diet and high- fat diet for 12 weeks respectively. Afterwards,the rats were sacrificed and their livers were weighed and preserved in liquid nitrogen. And eventually,the gene expression level of liver ferritin,hepcidin and transferrin were determined. Results Compared with control group,the gene expression level of liver ferritin and transferrin were all increased,while hepcidin gene expression level was decreased in obesity group,all with significant differences( P 0. 05). Conclusion Alterations of the expression level of genes,associated with liver iron protein( e. g. ferritin,hepcidin and transferrin),may cause iron malnutrition in obesity rats.