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Study on Glutathione S-transferase activity of blattella germanica in Pingshan

WU Song-lin;WU Neng-jian;CHEN Wei-wen;CHEN Jing-yang;LIU Xiao-na;WANG De-quan;Epidemiology Branch,Pingshan Center for Disease Control and Prevention of Shenzhen;  
Objective To study the activity of Glutathione S- transferases( GSTs) in the wild strains and sensitive strains of blattella germanica,inferring the resistant level,which provide the reference for further consummating biochemical resistance monitoring method and the selection of pesticides when prevent and control blattaria. Methods The enzyme activity was tested by spectrophotometry to find out the difference of Glutathione S- transferases between the different strains of blattella germanica. Results The Glutathione S- transferases activity of wild strains and sensitive strains blattella germanica were 19. 15 and 9. 14 U / mg prot,respectively,the GSTs activity of wild strains was significantly higher than that of the sensitive strains,the enzyme activity ratio( resistance coefficient) was 2. 10,and the difference was significant( t = 4. 33,P 0. 05). Conclusion From the biochemical level,the wild strains of blattella germanica which come from Pingshan is resistant,it may be closely related to the increase of the Glutathione S- transferases activity,which accelerates the ability of insecticide detoxification.
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