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《Chinese Journal of Public Health Engineering》 2017-01
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The similarities and differences of the gene of Beijing genotype M. tuberculosis between in Liaoning province and in Japan

SUN Lei;LIANG Jia-yuan;MAO Ning;LI Yue;SUN Guo-quan;WANG Cui;The Department for Tuberculosis Prevention and Control,Disease Prevention and Control Center of Liaoning Province;  
Objective To discuss the similarities and differences of the gene of Beijing genotype M. tuberculosis in Liaoning Province and in Japan. Methods PCR method was used to identify whether 144 clinical isolated Mycobacterium tuberculosis in Liaoning province and 84 Mycobacterium tuberculosis at the Kanagawa in Japan were Beijing genotype Mycobacterium tuberculosis or not. The DNA Bands amplified M. tuberculosis in Liaoning Province by primer 4 were sequenced and compared. Results In the 144 cases in liaoning province,Beijing genotype mycobacterium tuberculosis were 129 cases( 89. 58%). Non-Beijing genotype M. tuberculosis was 7 cases( 4. 86%). Beijing and non-Beijing genetype Mycobacterium tuberculosis mixed infection were 8 cases( 5. 56%),when primer 4 was used to amplify non-Beijing genotype M.tuberculosis,get the 308 bp bands. On the other hand,when primer 4 was used to amplify Beijing genotype M. tuberculosis in Liaoning province,there were many bands. The 270 bp band appear when 116 Beijing genotype Mycobacterium tuberculosis were amplified by the use of primer 4. The three 270 bp band of the 116 bands were sequenced in bidirectional sequencing. The sequencing results of the three 270 bp bands were same,while they were different from the sequence of308 bp bands. In the 84 cases at the Kanagawa in Japan,Beijing genotype mycobacterium tuberculosis were 53 cases( 63. 10%). Non-Beijing genotype M. tuberculosis was 31 cases( 36. 90%). When Primer 4 was used to amplify Beijing genotype M. tuberculosis at the Kanagawa in Japan,there was not the band. When primer 4 was used to amplify non-Beijing genotype M. tuberculosis,could get the 308 bp bands. Conclusion Beijing genotype Mycobacterium tuberculosis are the main popular in Liaoning Province. There are some multiple infections in the cases in Liaoning Province. There are the differences between the gene of Beijing genotype M. tuberculosis in Liaoning Province and that in Japan.
【Fund】: 辽宁省自然科学基金(2015020562)
【CateGory Index】: R378.911
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