Analysis of amino acid composition and the pharmacology of cordyceps sinensis
CHEN Li-yun;QI Zhen;Department of Medicine,The Fifth Affiliated Hospital of Sun Yat-sen University;
Objective To analyze the amino acid composition of Nordyceps pharmacology. Methods Cordyceps sinensis,which bred by the traditional Chinese medicine research institute,was routinely pretreated and powder extracted. The healthy male clean mice were trained at 7 weeks old,and the individuals with large differences were taken out. The powder was dried,baked,hydrolyzed and pretreated,followed by automatic amino acid analyzer. The contents of aspartic acid and serine were between 0.5% and 3.5%. 100 mice with no obvious difference in motor ability were randomly divided into 5 groups: model group,blank control group,high,middle and low dose amino group,except the model group,all of them were given intragastric administration for 4 weeks. Each time 0.4 ml,normal saline,model group only for training,not for oral administration,high,medium and low amino groups,respectively,given high,medium and low doses of amino acid0.4 ml,0.2 ml,0.1 ml. According to the proportion of amino acids mentioned above,after suspension in sodium carboxymethyl cellulose for 4 weeks,swimming exhaustion training was performed before the last administration,and continued to be administered by stomach for 30 minutes on 2 nd and at the last time. After swimming for 40 minutes,rest for 15 minutes,blood was taken from eyeball 0.1 ml,the content of lactic acid in whole blood was detected. In the same method,in addition to the blank control group,the other mice were injected cyclophosphamide 50 mg/kg intraperitoneally to make the model of immunosuppression in mice. 1 st once,two days in a row,after 2 weeks of gavage,the mice were given the last dose of cyclophosphamide for 20 minutes. Weighing,removing eyeball and blood,removing neck,removing spleen,weighing thymus,calculating viscera index,examining interleukin-2( IL-2),tumor necrosis factor α( TNF-α). Results In the amino acid group,the anti-fatigue ability of mice was significantly enhanced. the exhaustive swimming time increased from 0.7 min to 10.4 min. the swimming time of mice was significantly prolonged,while the serum lactic acid level decreased from 5.1 mmol/L to 1.3 mmol/L. meanwhile,the spleen index,thymus index,il-2,TNF-α levels were improved.The levels of lactic acid,spleen index,thymus index,interleukin-2( IL-2) and interleukin-α( TNF-α) in five groups of mice were statistically significant( P0.05),and the difference was statistically significant( P 0.05).Conclusion Cordyceps sinensis amino acids have anti-fatigue and immunity enhancement.
【CateGory Index】： R285.5