Variation analysis of amino acid site of VP1 coding region of enterovirus 71 in Dunhua city in 2009-2011
YANG Jin-hong;WEI Lei-lei;TIAN Li-min;WANG Shuang;TIAN Xin;CAO Feng-rui;CHENG Tao;SHAN Yuan-chun;FU Si-mei;LV Shu-mei;LI Da-qiang;Department of AIDS and Venereal Disease Control and Prevention,Dunhua Center for Disease Control and Prevention;
Objective To analyze the variation of amino acid site of VP1 coding region of enterovirus 71 in Dunhua city which cause hand food and mouth disease( HFMD) in 2009-2011. Methods 3 strains of EV-A71 virus isolated from the HFMD cases were used to do the RT-PCR and sequences of VP1 genes of EV-A71 strains,and were analyzed the gene phylogenetic and variation of amino acid site by the software of Mega4.0 and bioedit and.Results All 3 EV-A71 strains were belong to C4 a subgenotype in Dunhua city in 2009-2011,and the 3 strains had the same variation site from Q to H with those Jilin strains in the 22 th amino acid site in VP1 coding region,and also had the same variation with that Fuyang strain. Conclusion The EV-A71 which caused HFMD epidemic in Dunhua city in 2009-2011 has been spreading in other counties in Jilin province,and maybe direct or indirect from continuing transmission of Fuyang strains.
【CateGory Index】： R725.1