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《Chinese Journal of Animal Science》 2006-01
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Effects of Different Cryopreservation and Dilution Methods on the Development of Mouse Morulae

YANG Zhong-qiang1,ZHU Shi-en1,2*,ZHOU Guang-bin1,HOU Yun-peng1,YAN Chang-liang1(1.Laboratory of Animal Embryonic Biotechnology,College of Animal Science and Technology,China Agricultural uniuersity,Beijing 100094,China; 2.State Key Laboratories for Agrobiotechnology,China Agricultural University,Beijing 100094,China)  
Two slow freezing solutions and two vitrification solutions were used in the present study to cryopreserve Kunming mouse morulae,which were loaded in plastic straws.Embryos after slow freezing were diluted out of the straws while embryos after vitrification were diluted in the straws,and the effects of different cryopreservation and dilution method on the development of embryo were compared.After cultured in vitro,differences on the developmental rates(95.3%~95.8%,98.9%) between vitrification and slow freezing with 1.5 mol/L ethylene glycol were not significant(P 0.05).Embryos after slow freezing and vitrification with EFS30 and fresh embryos were respectively transferred to recipients.Differences between the rates of embryos that develope to live fetuses of the pregnant mice were not significant(50.8%,58.3%,54.9%;P 0.05).In conclusion,in-thawing is efficient when used on embryos after vitrification.It can be used as a technical parameter in practice.
【Fund】: 北京市农业科技重点项目(H022020060420)
【CateGory Index】: S814.8
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【References】
Chinese Journal Full-text Database 1 Hits
1 Liu Manqing~1,Yang Qien~1,Suo Lun~1,Liu Lin~1,Niu Ye~1,Zhu Shien~(1,2)(1.College of Animal Science and Technology,China Agricultural University,Beijing 100094,China;2.State key laboratory Agrobiotechnology,Beijing 100094,China);In-straw dilution and direct transfer of OPS vitrified mouse morulae[J];Journal of China Agricultural University;2006-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiu-fang, HUANG You-jun, HUANG Men-xiang, SHANG Jiang-hua(Institute of Buffalo Research,Chinese Academiy of Agriculture Sciences,Nanning 530001,China);Vitrification of buffalo embryos derived from in vitro fertilization[J];Herbivorous Animals;2003-01
2 Lei Ming Hua Zezhao(Institute of Refrigeration and Cryogenics,University of Shanghai of Science and Technology,Shanghai 200093)Kuang Yanping(Shanghai No.1 Hospital for Women and Infants,Shanghai);STUDY ON WORKING CONDITIONS UNDER WHICH SOLUTION EFSh40 VITRIFIES[J];Gryogenics;2001-06
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