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《Chinese Journal of Animal Science》 2019-01
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Bioinformatics and Tissue Expression Analysis of Splice Variants of Ovine Class Ⅱ IGF-Ⅰ Gene

SONG Xu-ting;ZHAI Yu-fei;ZHANG Jia-nan;QI Mei-yu;WANG Zhi-peng;FU Jing;YAO Yu-chang;Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province;College of Animal Science and Technology, Northeast Agricultural University;Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences;  
To identify their biological characteristics and functions in the process of growth and development of sheep IGF-Ⅰ splice variants, we successfully cloned the class Ⅱ IGF-Ⅰ gene splice variants(Ea and Eb), and utilized bioinformatics prediction webs to analysis the transcripts structure and detect their expression in different tissues. The results showed that sheep class Ⅱ-Ea and class Ⅱ-Eb IGF-Ⅰ gene, conceptually coding proteins of 138 aa and 172 aa, are newly cloned and from the same gene together with the class I-Ea in sheep. Bioinformatics analysis revealed that these two splice variants had same potential signal peptide cleavage sites, evolutionary homology, subcellular localization and protein spatial structure. But, they contained different numbers of covalent modification sites. Tissue expression analysis showed that all the two splice variants had the highest in the liver, followed by adipose, stomach, testis, muscle and skin, while in spleen, lung, kidney, intestine and bladder were low. In stomach, the expression level of two splice variants in lambs was higher than that of adult sheep(P0.01); In testis, the expression level of class Ⅱ-Ea IGF-Ⅰ in lambs was higher than that of adult sheep(P0.05). In general, the expression level of class Ⅱ-Ea IGF-Ⅰ gene in various tissues was higher than that of Eb type, which is the dominant transcriptional type.
【Fund】: 黑龙江省教育厅科学技术研究项目(12511034)
【CateGory Index】: S826
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