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《Chinese Journal of Animal Science》 2019-01
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Study on the Relationship between Nutritional Value, Ruminal Degradation Characteristics and Molecular Structure of Protein in Different Heat-treated DDGS

XU Hong-jian;LI Xin;YAO En-yue;ZHEGN Jian;ZHAO Hong-bo;ZHANG Yong-gen;College of Animal Science and Technology, Northeast Agricultural University;Center of Practice and Demonstration, Northeast Agricultural University;  
The aim of the experiment was to investigate the correlation between protein molecular structure and protein chemical compositions, CNCNPS compositions, ruminal degradability and intestinal digestibility in different heat-treated dried distillers grains with solubles(DDGS). Cornell net carbohydrate protein system, nylon bag technology and three-step in vitro method were used to evaluate the utilization value of different heat treated DDGS. Fourier transform infrared spectroscopy(FTIR) was used to analyze the changes of DDGS protein molecular structure, and then relationship between both was researched. The results showed that the heating reduced the ruminal degradability and intestinal digestibility of DDGS and increased the digestible protein in the intestine. Different heat treatments had a significant effect on the molecular structure of DDGS protein(P0.05). The amide I band peak height, peak area, amide II band peak height, peak area, ratio of amide I band and amide II band peak height and peak area in protein molecular structure were significantly correlated to the nutritional value of protein in DDGS, ruminal degradation parameters and intestinal digestibility(P0.05). In summary, heat treatments have an impact on the molecular structure, nutritional value, ruminal degradability and intestinal digestibility of protein. Protein molecular structure of DDGS can effectively estimate protein nutritional valu e, ruminal degradability, and small intestine digestibility. FTIR spectroscopy can be used to evaluate the nutritional value and heating degree of DDGS.
【Fund】: 国家奶牛产业技术体系(CARS-36)
【CateGory Index】: S816
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