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《China Pharmacy》 2010-47
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Effect of 7 Kinds of Active Constituents from Astragalus membranaceus on the Expression of HIF-1

LIANG Li-juan, ZHAO Kui-jun(Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China) DONG Ting-xia, TSIM Wahkeung Karl(Dept. of Biology and Center for Chinese Medicine Research, Hong Kong University of Science and Technology, HongKong 999075, China) TU Peng-fei(School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China)  
OBJECTIVE: To explore the effect of formononetin,ononin,calycosin-O-β-D-glucoside,calycosin,acetylastragalosideⅠ,astragalosideⅡ, astragaloside Ⅳ from Astragalus membranaceus on the expression of hypoxia-induced factor-1(HIF-1). METHODS: The HRE, derived from EPO containing a highly conserved HIF-1 binding site, were cloned into pBI-GL vectors that contained luciferase reporter genes. The HEK-293T cells were transfected cDNA constructs by calcium phosphate precipitation methods. The activity of luciferase was determined after 48 h treatment. The expression of HIF-1 was evaluated using luciferase report gene system. RESULTS:10 μmol·L-1 formononetin promote expression of HIF-1.Ononin had an effect on the expression of HIF-1 in a concentration-dependent manner. Low concentration could elevate the expression of HIF-1 while high concentration down-regulated the expression. Other 5 monomers had no obvious effect on the expression of HIF-1. CONCLUSION:The study provide reference for the anti-tumor effect of A. membranaceus and therapeutic effect of it for other disease.
【Fund】: 国家重点基础研究发展计划(973计划)课题(2006CB504707)
【CateGory Index】: R285.5
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Chinese Journal Full-text Database 1 Hits
1 MAO Jian,LI Rong-heng(Dept. of Traditional Chinese and Western Medicine,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China);Effect of Fuyuan Capsule on the Expression of HIF-1α Kidney of Sepsis Rats[J];China Pharmacy;2010-15
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 AO Qi-lin1, HUANG Lei2, ZHU Peng-cheng1, WANG Wei1, WANG Di-xun3 (1Department of Pathology,2Department of Gynecology and Obstetrics of Tongji Hospital,3Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);The mechanism of lipopolysaccharide-activated rat alveolar macrophages producing tumor necrosis factor alpha[J];Chinese Journal of Pathophysiology;2006-01
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