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IMPROVEMENT AND APPLICATION OF ANALYTICAL METHOD FOR TOTAL GLUCOSINOLATE CONTENTS IN RAPE

Peng Liangcai Wu Xinyong Xu Runfang (Institute of Oil Crops Research,CAAS)  
A thiourea-UV assay for total glucosinolate contents was improved,mainly suitable for the determining that in seeds and plant organs in rape.On the basis of the results investigated by Wetter,Guo Nanjing and Wu xinyong,the main programmes for analysing glucosinolates with myrosinase hydrolysis were modified and the applied condition of this method was studied in order to shorten the time needed for extracting and purifing myrosinase,avoid the restriction of hydrolysis only depends on thioglucoside glucohydrolase in rape,and enlarge determination range.
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