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Effect of Manganese Oxidative Stress on Contents of Cordycepin and Adenosine in Mycelia of Cordyceps militaris

ZUO Yan-mei;CHENG Xian-hao;ZHU Meng;LI Wei-huan;College of Life Science,Ludong University;  
OBJECTIVE To explore the impact of of manganese(Mn) ions stress on the contents of cordycepin and adenosine in mycelia of C.militaris.METHODS The mycelia biomass,Mn accumulation and activities of antioxidant enzymes [catalase(CAT),peroxidase(POD) and superoxide dismutase(SOD) ] in mycelia of C.militaris with liquid culture medium supplemented with Mn2 + from 0 to 100 g·L-1were determined.The contents of cordycepin and adenosine in mycelia under different manganese oxidative stress were determined by HPLC.The separation was performed on a Zorbax Extend-C 18 column(4.6 mm × 150 mm,4 μm),using acetonitrile-water(5∶ 95) as the mobile phase.The flow rate was 1.0 mL·min-1.The detection wavelength was set at 254 nm.RESULTS The mycelia biomass of C.militaris decreased with the increase of manganese concentration.The manganese content in mycelia increased significantly with the increase of manganese concentration and reached plateau when the manganese concentration was higher than 30 g·L-1.The activity of antioxidant enzymes including POD,CAT and SOD in mycelia of C.militaris all increased first and then decreased with increasing concentration of manganese in the culture media.The cordycepin content in mycelia increased significantly with increase of manganese concentration.When the Mn2 +concentration in the culture medium was 80 g·L-1,cordycepin content in mycelia was 3.31 times higher than the control.The adenosine content decreased significantly when the manganese concentration was in the range of 0-30 g·L-1,and then remained relatively stable.CONCLUSION Oxidative stress of manganese ions promotes the cordycepin synthesis in mycelia of C.militaris,but inhibites adenosine synthesis.
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