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《Chinese Journal of Clinical Oncology and Rehabilitation》 2018-09
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Relationship between composition of vaginal microbial community and precancerous cervical lesions in women

ZHOU Ke;MA Teng-yuan;XING Guo-zheng;GAN Lu;Department of Clinical Laboratory,Beijing University of Traditional Chinese Medicine Affiliated Huguosi Hospital of Traditional Chinese Medicine;Department of Clinical Laboratory,Beijing Xicheng District Desheng Community Health Service Center;Department of Clinical Laboratory,Beijing Aerospace General Hospital;Department of Clinical Laboratory,Beijing Xuanwu Hospital of Traditional Chinese Medicine;  
Objective to study the relationship composition of vaginal microbial community and precancerous cervical lesions in women. Methods A total of 100 women admitted to Beijing Huguosi Hospital of Traditional Chinese Medicine,Beijing Xicheng District Desheng Community Health Service Center,Beijing Aerospace General Hospital,and Beijing Xuanwu Hospital of Traditional Chinese Medicine from January 2013 to December 2017 were selected as the study subjects for the retrospective analysis. They were divided into three groups,among which 28 women with cervical intraepithelial neoplasia I were included in CIN I group,38 women with cervical intraepithelial neoplasiaⅡ/Ⅲ were included in CINⅡ/Ⅲ group,and of 34 healthy women who had physical examination were included in the healthy group. Bacterial genomic DNA was extracted from vaginal secretions in all women. Using PCR technology to amplify 16 S rRNAV3 and V4 area gene segment,and the Illumina Miseq sequencing platform was used for amplified PCR product sequencing. Diversity and abundance of vaginal microbial community species were assessed,and bacteria microbial community and composition of each sample were compared. Results For the Alpha indexes there was no significant difference between the healthy group and CIN Ⅱ/Ⅲ grouop( P 0. 05). However,Chao value and ACE value in CIN I group were significantly higher than in the healthy group( all P 0. 05).Among the detected 23 bacterium,the number of Firmicutes were lower in CIN I grouop than in the other tow groups and the number of Actinobacteria were higher than the other groups. There were significant difference in the detection rate among the three groups( all P 0. 05). The number of Lactobacillus was significant lower in the CIN I group than in the healthy group and CIN Ⅱ/Ⅲ group. The number of Gardnerella was significant lower in the CIN Ⅱ/Ⅲ group than in the CIN I group. The number of Atopobium was significant lower in the CIN I group than other two groups. There were significant differences in the distribution of vaginal microflora among the three groups( all P 0. 05). Conclusion In patients with different degrees of cervical intraepithelial neoplasia,the diversity and flora types of vaginal microflora are roughly the same as those of healthy women,but some bacterial flora abundance has changed,mainly due to the decrease of Lactobacillus genus and the increase in the levels of Gardneria and Atopobium. This suggests that changes in the composition of vaginal microbiome may be strongly associated with the development of cervical cancer lesions.
【CateGory Index】: R737.33
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